Kohlmeier M
Clin Chem. 1986 Jan;32(1 Pt 1):63-6.
A simple two-step procedure is now available for directly measuring triglycerides in whole serum and in lipoprotein fractions. I tested the performance of the assay, using pooled serum stored frozen, freshly prepared lipoprotein fractions, and pure glyceride standards. A parabolic concentration-response curve ensured linearity well beyond total glycerol concentrations of 10 mmol/L, but gave rise to misleading results for massively lipemic samples. Within-day CVs averaged 2.2% for the stored-frozen serum pools; mean day-to-day variation was 2.4%. Analytical recoveries of triglycerides, after lipoprotein fractionation, ranged between 101.9 and 103.7%. Aqueous glyceride standards gave results equivalent to between 95 and 105% of their glycerol content.
现在有一种简单的两步法可用于直接测定全血清和脂蛋白组分中的甘油三酯。我使用冷冻保存的混合血清、新鲜制备的脂蛋白组分和纯甘油酯标准品测试了该测定方法的性能。一条抛物线形的浓度-响应曲线确保了线性范围远超过10 mmol/L的总甘油浓度，但对于严重脂血样本会产生误导性结果。冷冻保存的血清池日内变异系数平均为2.2%；日间平均变异为2.4%。脂蛋白分级分离后甘油三酯的分析回收率在101.9%至103.7%之间。水性甘油酯标准品给出的结果相当于其甘油含量的95%至105%。
