The Shmunis School of Biomedicine and Cancer Research, Faculty of Life Sciences, Tel Aviv University, 6997801, Tel Aviv, Israel.
The Center for Nanoscience and Nanotechnology, Tel Aviv University, 6997801, Tel Aviv, Israel.
J Nanobiotechnology. 2022 Jan 31;20(1):59. doi: 10.1186/s12951-022-01261-8.
Controlled release systems are often integrated into polymeric scaffolds to supply essential biofactors to trigger physiological processes in engineered tissues. Here, we report the modification of chondroitin sulfate (CS) electroactive polymer with gold nanorods (AuNRs) to create hybrid macroporous scaffolds for enhanced on-demand release of growth factors and cytokines. The mechanical properties, porosity and degradation of the hybrid scaffolds were evaluated, and the viability and functionality of seeded cardiac cells were assessed. Following, the ability to control the release of the enzyme lysozyme, and the cytokine, stromal cell-derived factor 1 (SDF-1) by applying electrical stimulation, was demonstrated. The AuNRs were able to increase the current through the scaffolds, providing an efficient on-off release profile of SDF-1, which resulted in higher migration of cells expressing CXCR4 receptor. Finally, the engineered scaffolds were transplanted in rats and SDF-1 was released daily by electrical stimulation, promoting blood vessel-forming cell infiltration and vascularization. We envision that gold nanoparticles and other conducting nanomaterials can be incorporated into different electroactive materials to improve their capabilities not only for tissue engineering applications, but for a variety of biomedical applications, where enhanced electrical stimulation is needed.
控释系统通常被整合到聚合物支架中，以提供必需的生物因素来触发工程组织中的生理过程。在这里，我们报告了金纳米棒（AuNRs）修饰的硫酸软骨素（CS）电活性聚合物，以创建用于增强按需释放生长因子和细胞因子的混合大孔支架。评估了混合支架的机械性能、孔隙率和降解情况，并评估了接种的心脏细胞的活力和功能。接下来，通过施加电刺激，证明了控制酶溶菌酶和细胞因子基质细胞衍生因子 1（SDF-1）释放的能力。AuNRs 能够增加通过支架的电流，提供 SDF-1 的高效开/关释放曲线，从而导致表达 CXCR4 受体的细胞更高的迁移。最后，将工程支架移植到大鼠体内，并通过电刺激每天释放 SDF-1，促进血管生成细胞的浸润和血管生成。我们设想可以将金纳米粒子和其他导电纳米材料纳入不同的电活性材料中，以提高它们的性能，不仅可用于组织工程应用，还可用于各种需要增强电刺激的生物医学应用。
