The Laboratory for Tissue Engineering and Regenerative Medicine, Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Science, ‡The Center for Nanoscience and Nanotechnology, and §Department of Materials Science and Engineering, Tel Aviv University , Tel Aviv 69978, Israel.
Nano Lett. 2014 Oct 8;14(10):5792-6. doi: 10.1021/nl502673m. Epub 2014 Sep 8.
Decellularized matrices are valuable scaffolds for engineering functional cardiac patches for treating myocardial infarction. However, the lack of quick and efficient electrical coupling between adjacent cells may jeopardize the success of the treatment. To address this issue, we have deposited gold nanoparticles on fibrous decellularized omental matrices and investigated their morphology, conductivity, and degradation. We have shown that cardiac cells engineered within the hybrid scaffolds exhibited elongated and aligned morphology, massive striation, and organized connexin 43 electrical coupling proteins. Finally, we have shown that the hybrid patches demonstrated superior function as compared to pristine patches, including a stronger contraction force, lower excitation threshold, and faster calcium transients.
去细胞基质是构建用于治疗心肌梗死的功能性心脏贴片的有价值的支架。然而，相邻细胞之间缺乏快速有效的电偶联可能会危及治疗的成功。为了解决这个问题，我们在纤维状去细胞大网膜基质上沉积了金纳米粒子，并研究了它们的形态、导电性和降解性。我们已经表明，在混合支架中构建的心脏细胞表现出伸长和对齐的形态、大量的条纹和有组织的连接蛋白 43 电偶联蛋白。最后，我们已经表明，与原始贴片相比，混合贴片表现出更好的功能，包括更强的收缩力、更低的兴奋阈值和更快的钙瞬变。
