Xie Tianqu, Wang Yuchen, Zou Zhijie, Wu Yinyin, Fan Xiaoxiao, Dai Jiamiao, Liu Yanqun, Bai Jinbing
Wuhan University School of Nursing, Wuhan University, 169 Donghu Road, Wuhan, 430071, China.
Taiyuan Central Hospital of Shanxi Medical University, No. 5 Dong San Dao Xiang, Jiefang Road, Taiyuan, 030009, China.
J Psychiatr Res. 2022 Apr;148:52-60. doi: 10.1016/j.jpsychires.2022.01.041. Epub 2022 Jan 24.
Understanding the relationship between the gut microbiota and temperament can provide new insights for the regulation of behavioral intervention in children, which is still lacking research. This study aimed to examine the relationship between the gut microbiota and temperament in a cohort of children in 1 year and 2 years old.
This study included a total of 37 children with completed information, in which 51 samples at age 1 and 41 samples at age 2 were received respectively. We collected birth and demographic information. Parents reported their child's temperament characteristics using the Infant Behavior Questionnaire-revised (IBQ-R) and Early Childhood Behavior Questionnaire (ECBQ). Fecal samples were collected from each child at 1 and 2 years old and sequenced with MiSeq sequencer. Multiple linear regressions and linear mixed effect models were used to analyze the relationship between the temperament and their microbiota composition as well as the diversity and effect of gender or age on this relationship.
At age of year 2, Faecalibacterium was negatively associated with high-intensity pleasure and surgency. Bifidobacterium was negatively correlated with Perceptual sensitivity. Results showed no difference about three domains between year 1 and year 2, while gut microbiota showed diversity difference and genera difference. There was no gender and age difference on the relationship between temperament and the gut microbiota.
Temperament was associated with the gut microbiota over time. The temperament remained stable and the relationship between the gut microbiota and temperament wasn't associated with age and gender.
了解肠道微生物群与气质之间的关系可为儿童行为干预的调节提供新的见解，但目前仍缺乏相关研究。本研究旨在探讨1岁和2岁儿童队列中肠道微生物群与气质之间的关系。
本研究共纳入37名信息完整的儿童，分别采集了51份1岁时的样本和41份2岁时的样本。我们收集了出生和人口统计学信息。父母使用修订版婴儿行为问卷（IBQ-R）和幼儿行为问卷（ECBQ）报告孩子的气质特征。在1岁和2岁时从每个孩子采集粪便样本，并用MiSeq测序仪进行测序。使用多元线性回归和线性混合效应模型分析气质与其微生物群组成之间的关系，以及性别或年龄对这种关系的多样性和影响。
在2岁时，粪杆菌与高强度愉悦感和活泼性呈负相关。双歧杆菌与感知敏感性呈负相关。结果显示，1岁和2岁时三个维度没有差异，而肠道微生物群存在多样性差异和属差异。气质与肠道微生物群之间的关系没有性别和年龄差异。
随着时间的推移，气质与肠道微生物群有关。气质保持稳定，肠道微生物群与气质之间的关系与年龄和性别无关。
