Khaje Roshanaee Mohammad, Abtahi-Eivary Seyed Hosein, Shokoohi Majid, Fani Masoumeh, Mahmoudian Azamsadat, Moghimian Maryam
Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran.
Department of Biochemistry, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.
Int J Fertil Steril. 2022 Jan;16(1):30-35. doi: 10.22074/IJFS.2021.522550.1069.
Minocycline is a widely used bacteriostatic antibiotic with various functions. The aim of this study was to investigate impact of apoptotic genes in ovary of the torsion/detorsion treated rat model by minocycline.
This experimental study was performed in 32 female Wistar rats classified in four groups, including: i. sham, ii. TD: torsion/detorsion group received normal saline, iii. TDM: torsion/detorsion group treated with 40 mg/kg Minocycline, and iv. MC: healthy group received 40 mg/kg Minocycline. After treatment period (7 days), histoplogical parameters, oxidative stress markers and hormone profile of serum as well as the expression of and genes were measured in the ovary of rats.
Levels of superoxide dismutase (SOD), glutathione peroxidase (GPX) and estrogen were decreased in the TD group and significantly increased in the treated groups (P=0.001). Levels of malondialdehyde (MDA) and testosterone were increased in the TD group and decreased in the treated groups (P=0.001). Expression level of was elevated in the TD group, while it was attenuated in the treated groups (P=0.001). Expression level of was significantly increased in treated groups (P=0.001).
Minocycline can repair oxidative damage in ovarian tissue and regulate apoptotic-related gene expressions.
米诺环素是一种广泛使用的具有多种功能的抑菌抗生素。本研究的目的是探讨米诺环素对扭转/复位处理的大鼠卵巢凋亡基因的影响。
本实验研究在32只雌性Wistar大鼠中进行，分为四组，包括：i. 假手术组；ii. TD：扭转/复位组，给予生理盐水；iii. TDM：扭转/复位组，用40mg/kg米诺环素处理；iv. MC：健康组，给予40mg/kg米诺环素。治疗期（7天）后，测量大鼠卵巢的组织学参数、氧化应激标志物、血清激素水平以及 和 基因的表达。
TD组超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）和雌激素水平降低，治疗组显著升高（P = 0.001）。TD组丙二醛（MDA）和睾酮水平升高，治疗组降低（P = 0.001）。TD组 的表达水平升高，而治疗组则减弱（P = 0.001）。治疗组 的表达水平显著升高（P = 0.001）。
米诺环素可修复卵巢组织的氧化损伤并调节凋亡相关基因的表达。
