Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran; Young Research Societies, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran.
Chemosphere. 2022 May;295:133869. doi: 10.1016/j.chemosphere.2022.133869. Epub 2022 Feb 5.
Melatonin (MLT), a hormone related to the regulation of brain functions, is directly related to sleep quality and is considered to be a possible adjuvant therapy for patients needing hospitalization for coronavirus disease 2019 pneumonia, and accurate measurement of MLT is crucial. Herein, a new, highly sensitive, and easy operation fluorescent probe was provided based on Zr metal-organic framework encapsulation into the molecularly imprinted polymer (MOF@MIP). By combining unique properties of MIP and fluorescent MOF, selectivity and operation of the applied method were significantly improved. Different characterization methods, such as XRD, FT-IR, and FE-SEM, were used to confirm the synthesis reliability. MOF@MIP was successfully used for the precise identification and ultrasensitive detection for trace amounts of MLT. The detection mechanism for the analytical system is based on the ''turn-on'' fluorescence (FL) signal in 404 nm. The findings proved that it is possible to detect trace amounts of MLT in real samples including grape, cherry, and sour cherry juice. The linear range and the limit of detection (LOD) for trace amounts of MLT were obtained as 1-100 ng/mL and 0.18 ng/mL, respectively.
褪黑素（MLT）是一种与大脑功能调节相关的激素，与睡眠质量直接相关，被认为是 COVID-19 肺炎住院患者的一种可能的辅助治疗方法，因此准确测量 MLT 至关重要。在此，提供了一种基于 Zr 金属有机骨架（MOF）封装到分子印迹聚合物（MIP）中的新型、高灵敏度、易于操作的荧光探针。通过结合 MIP 和荧光 MOF 的独特性质，显著提高了应用方法的选择性和操作性。使用不同的表征方法，如 XRD、FT-IR 和 FE-SEM，来确认合成的可靠性。MOF@MIP 成功用于痕量 MLT 的精确识别和超灵敏检测。分析系统的检测机制基于在 404nm 处的“开启”荧光（FL）信号。研究结果证明，有可能检测到真实样品（包括葡萄、樱桃和酸樱桃汁）中的痕量 MLT。痕量 MLT 的线性范围和检测限（LOD）分别为 1-100ng/mL 和 0.18ng/mL。
