Aguilera A, Benítez T
Arch Microbiol. 1986 Jan;143(4):337-44. doi: 10.1007/BF00412799.
Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21 selected monogenic ethanol-sensitive mutants define 20 complementation groups, denominated ETA1 to ETA20, which indicates that there is a high number of genes involved in the ethanol tolerance/sensitivity mechanism. Out of 21 selected monogenic mutants, 20 are not altered in the glycolytic pathway since, when maintained in glucose-supplemented medium, they can produce as much ethanol as the wild type and at about the same velocity. Nor do any of the mutants seem to be altered in the lipid biosynthetic pathway since, whether grown in the absence or in the presence of ethanol, their concentration of fatty acids and ergosterol is similar to that of the wild type under the same conditions. Therefore growth sensitivity to ethanol does not seem necessarily to be related to carbohydrate or lipid metabolism.
已经分离出酿酒酵母突变体，这些突变体在野生型菌株S288C能够生长的乙醇浓度下无法生长。其中一些突变体在菌落大小、温度敏感性和在乙醇中的活力方面表现出额外的表型改变，这些改变与对乙醇的生长敏感性共分离。21个选定的单基因乙醇敏感突变体定义了20个互补群，命名为ETA1至ETA20，这表明有大量基因参与乙醇耐受性/敏感性机制。在21个选定的单基因突变体中，20个在糖酵解途径中没有改变，因为当在补充葡萄糖的培养基中培养时，它们能够产生与野生型一样多的乙醇，且速度大致相同。这些突变体似乎也没有在脂质生物合成途径中发生改变，因为无论在有无乙醇的情况下生长，它们的脂肪酸和麦角固醇浓度在相同条件下都与野生型相似。因此，对乙醇的生长敏感性似乎不一定与碳水化合物或脂质代谢有关。
