Huang Jia-Bin, Hu Bin-Bin, He Rong, He Lian, Zou Chen, Man Chang-Feng, Fan Yu
Cancer Institue, Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.
Front Genet. 2022 Jan 24;12:787800. doi: 10.3389/fgene.2021.787800. eCollection 2021.
From previous studies, we found that there are more than 100 types of RNA modifications in RNA molecules. mA methylation is the most common. The incidence rate of adenocarcinoma of the esophagogastric junction (AEG) at home and abroad has increased faster than that of stomach cancer at other sites in recent years. Here, we systematically analyze the modification pattern of mA mRNA in adenocarcinoma at the esophagogastric junction. mA sequencing, RNA sequencing, and bioinformatics analysis were used to describe the mA modification pattern in adenocarcinoma and normal tissues at the esophagogastric junction. In AEG samples, a total of 4,775 new mA peaks appeared, and 3,054 peaks disappeared. The unique m6A-related genes in AEG are related to cancer-related pathways. There are hypermethylated or hypomethylated mA peaks in AEG in differentially expressed mRNA transcripts. This study preliminarily constructed the first mA full transcriptome map of human AEG. This has a guiding role in revealing the mechanism of mA-mediated gene expression regulation.
从以往的研究中，我们发现RNA分子中存在100多种RNA修饰类型。N6-甲基腺嘌呤（mA）甲基化最为常见。近年来，国内外食管胃交界腺癌（AEG）的发病率增长速度快于其他部位的胃癌。在此，我们系统地分析了食管胃交界腺癌中mA mRNA的修饰模式。采用mA测序、RNA测序和生物信息学分析来描述食管胃交界腺癌和正常组织中的mA修饰模式。在AEG样本中，共出现4775个新的mA峰，3054个峰消失。AEG中独特的m6A相关基因与癌症相关通路有关。差异表达的mRNA转录本中，AEG存在甲基化过高或过低的mA峰。本研究初步构建了人类AEG的首个mA全转录组图谱。这对揭示mA介导的基因表达调控机制具有指导作用。
