Torres-Garcia Daniel, Gené Josepa, García Dania
Universitat Rovira i Virgili, Unitat de Micologia, Facultat de Medicina i Ciències de la Salut and IISPV, 43201-Reus, Spain Universitat Rovira i Virgili Reus Spain.
MycoKeys. 2022 Feb 1;86:103-145. doi: 10.3897/mycokeys.86.73861. eCollection 2022.
species are common fungi found worldwide from diverse substrates, including soil, plant debris, food products and air. Their diversity in aquatic environments is still underexplored. With the aim to explore the fungal diversity in Spanish freshwater sediments, numerous strains were isolated using various culture-dependent techniques. A preliminary sequence analysis of the β-tubulin (2) gene marker allowed us to identify several interesting species of , which were later characterized phylogenetically with the barcodes recommended for species delimitation in the genus. Based on the multi-locus phylogeny of the internal transcribed spacer region (ITS) of the ribosomal DNA, and partial fragments of 2, calmodulin (), and the RNA polymerase II largest subunit (2) genes, in combination with phenotypic analyses, five novel species are described. These are in sectionLanata-Divaricata, in sect.Gracilenta, in sect.Canescentia, in sect.Paradoxa and in sect.Robsamsonia. The study of several isolates from samples collected in different locations resulted in the reinstatement of into sectionCitrina. Finally, (sect.Exilicaulis) and (sect.Chrysogena) are reported, previously only known from Antarctica and China, respectively.
这些物种是常见真菌，在全球范围内的各种基质中都能找到，包括土壤、植物残体、食品和空气。它们在水生环境中的多样性仍未得到充分探索。为了探索西班牙淡水沉积物中的真菌多样性，使用了各种依赖培养的技术分离出了许多菌株。对β-微管蛋白（2）基因标记进行初步序列分析，使我们能够鉴定出几种有趣的物种，随后利用推荐用于该属物种界定的条形码对其进行了系统发育特征分析。基于核糖体DNA内部转录间隔区（ITS）以及2、钙调蛋白（）和RNA聚合酶II最大亚基（2）基因的部分片段的多位点系统发育分析，并结合表型分析，描述了五个新物种。它们分别是节兰纳塔-迪瓦里卡塔中的、节格拉西伦塔中的、节坎森蒂亚中的、节悖论中的和节罗布萨姆索尼娅中的。对从不同地点采集的样本中分离出的几种菌株进行研究后，将重新归入节柠檬黄。最后，报告了（节埃西利卡利斯）和（节金黄），它们此前分别仅在南极洲和中国被发现。
