New and interesting species of (Eurotiomycetes, Aspergillaceae) in freshwater sediments from Spain.

species are common fungi found worldwide from diverse substrates, including soil, plant debris, food products and air. Their diversity in aquatic environments is still underexplored. With the aim to explore the fungal diversity in Spanish freshwater sediments, numerous strains were isolated using various culture-dependent techniques. A preliminary sequence analysis of the β-tubulin (2) gene marker allowed us to identify several interesting species of , which were later characterized phylogenetically with the barcodes recommended for species delimitation in the genus. Based on the multi-locus phylogeny of the internal transcribed spacer region (ITS) of the ribosomal DNA, and partial fragments of 2, calmodulin (), and the RNA polymerase II largest subunit (2) genes, in combination with phenotypic analyses, five novel species are described. These are in sectionLanata-Divaricata, in sect.Gracilenta, in sect.Canescentia, in sect.Paradoxa and in sect.Robsamsonia. The study of several isolates from samples collected in different locations resulted in the reinstatement of into sectionCitrina. Finally, (sect.Exilicaulis) and (sect.Chrysogena) are reported, previously only known from Antarctica and China, respectively.