Machado Edilson S, Soares Fabiano P, Yamaguchi Roberta S, Felipone William K, Meves Robert, Souza Tais Amara C, Topolniak Roberto, Caldas José P, Abreu Ernani V, Rabelo Neto Luiz S, Pinchemel Pedro Vinicius S, Bredemeier Markus
Pain Medical Center, Regenerar, Porto Alegre, BRA.
Faculty of Medicine, University of Porto, Porto, PRT.
Cureus. 2022 Jan 3;14(1):e20899. doi: 10.7759/cureus.20899. eCollection 2022 Jan.
To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use in clinical practice and compare this technique with methods that have been previously described in the medical literature.
Sixteen blood samples from healthy volunteers were collected. PRP was prepared using our new double-spin technique, consisting of successive centrifugation of blood samples with two different spins, without opening the container. Descriptive analysis of cell counts in baseline and PRP samples was undertaken. Comparison between cell and platelet count in baseline and PRP samples, as well as the statistical analysis, were done.
The mean platelet concentration ratio was 3.47 (SD: 0.85; 95% CI: 3.01-3.92; range: 2.48-5.71). The baseline whole blood platelet count correlated positively to the PRP platelet count (rP= 0.56; 95% CI: 0.09-0.88; P = 0.023). The PRP was enriched for lymphocytes and monocytes but presented significantly lower counts of neutrophils and eosinophils in comparison to baseline.
Results show a safe and easily reproducible method to obtain PRP for use in clinical daily practice.
描述并分析一种用于临床实践的富血小板血浆（PRP）提取新方案，并将该技术与医学文献中先前描述的方法进行比较。
采集了16名健康志愿者的血样。使用我们的新双旋技术制备PRP，该技术包括在不打开容器的情况下对血样进行连续两次不同转速的离心。对基线样本和PRP样本中的细胞计数进行描述性分析。对基线样本和PRP样本中的细胞和血小板计数进行比较，并进行统计分析。
平均血小板浓度比为3.47（标准差：0.85；95%置信区间：3.01 - 3.92；范围：2.48 - 5.71）。基线全血血小板计数与PRP血小板计数呈正相关（rP = 0.56；95%置信区间：0.09 - 0.88；P = 0.023）。与基线相比，PRP中淋巴细胞和单核细胞增多，但中性粒细胞和嗜酸性粒细胞计数显著降低。
结果表明，该方法是一种安全且易于重复的获取用于临床日常实践的PRP的方法。
