Gálvez Alvaro Tapia, Cordova Abel Teves, Juarez Vilcapuma Oniel Elías, Atoche-Socola Katherine Joselyn, Sánchez Sergio Jiménez
School of Dentistry, Universidad Científica Del Sur, Lima Perú.
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
J Lasers Med Sci. 2024 Nov 25;15:e57. doi: 10.34172/jlms.2024.57. eCollection 2024.
In recent years, various methods such as lasers and different solutions have been investigated to eliminate microorganisms in the root canal. This investigation was aimed at comparing the bactericidal effect on between a diode laser with a wavelength of 940 nm and a mixture of a tetracycline isomer, an acid, and a detergent (MTAD). Forty single-rooted human lower premolar teeth were prepared for inoculation with and incubated for a period of 3 weeks. The 40 samples were randomly divided into 4 groups: the 940 nm diode laser group with irradiation through a 200 µm optical fiber with parameters of (1 W/CW,4 cycles, 20s interval); the MTAD group irrigated with 5 mL for 5 minutes; also, a positive group with 3% NaOCl and a negative group with 0.9% saline solution, both irrigated with 5 mL for 5 minutes. For statistical analysis, the Mann-Whitney U and Wilcoxon tests were used. The 3% NaOCl positive control group had greater bacterial reduction after treatment (99.99%) compared to the 940nm diode laser (98.96%) and MTAD (99.34%) groups. Statistically, we found a significant difference between all groups regarding the reduction in the colony count (<0.05). It was found that there was a significant difference in the percentage of reduction for the bacterial colonies between all the groups evaluated, with the greatest antibacterial effectiveness observed with 3% sodium hypochlorite.
近年来,人们研究了多种方法,如激光和不同的溶液,以消除根管内的微生物。本研究旨在比较波长为940nm的二极管激光与四环素异构体、酸和洗涤剂的混合物(MTAD)的杀菌效果。选取40颗单根人下颌前磨牙,制备后接种细菌并培养3周。将40个样本随机分为4组:940nm二极管激光组,通过200μm光纤照射,参数为(1W/连续波,4个周期,间隔20s);MTAD组用5mL冲洗5分钟;还有3%次氯酸钠阳性对照组和0.9%生理盐水阴性对照组,均用5mL冲洗5分钟。统计分析采用Mann-Whitney U检验和Wilcoxon检验。与940nm二极管激光组(98.96%)和MTAD组(99.34%)相比,3%次氯酸钠阳性对照组治疗后的细菌减少率更高(99.99%)。统计学上,我们发现所有组在菌落计数减少方面存在显著差异(<0.05)。结果发现,所有评估组的细菌菌落减少百分比存在显著差异,3%次氯酸钠的抗菌效果最佳。