Thomas Stacey M, Ackert-Bicknell Cheryl L, Zuscik Michael J, Payne Karin A
Colorado Program for Musculoskeletal Research, Department of Orthopedics, University of Colorado Anschutz Medical Campus, Mail Stop 8343, 12800 East 19th Avenue, Aurora, CO, 80045, USA.
Gates Center for Regenerative Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Curr Osteoporos Rep. 2022 Apr;20(2):141-152. doi: 10.1007/s11914-022-00726-x. Epub 2022 Feb 14.
RNA-sequencing (RNA-seq) is a novel and highly sought-after tool in the field of musculoskeletal regenerative medicine. The technology is being used to better understand pathological processes, as well as elucidate mechanisms governing development and regeneration. It has allowed in-depth characterization of stem cell populations and discovery of molecular mechanisms that regulate stem cell development, maintenance, and differentiation in a way that was not possible with previous technology. This review introduces RNA-seq technology and how it has paved the way for advances in musculoskeletal regenerative medicine.
Recent studies in regenerative medicine have utilized RNA-seq to decipher mechanisms of pathophysiology and identify novel targets for regenerative medicine. The technology has also advanced stem cell biology through in-depth characterization of stem cells, identifying differentiation trajectories and optimizing cell culture conditions. It has also provided new knowledge that has led to improved growth factor use and scaffold design for musculoskeletal regenerative medicine. This article reviews recent studies utilizing RNA-seq in the field of musculoskeletal regenerative medicine. It demonstrates how transcriptomic analysis can be used to provide insights that can aid in formulating a regenerative strategy.
RNA测序（RNA-seq）是肌肉骨骼再生医学领域一种新颖且备受追捧的工具。该技术正被用于更好地理解病理过程，以及阐明发育和再生的调控机制。它使得干细胞群体能够得到深入表征，并发现了调节干细胞发育、维持和分化的分子机制，这是以往技术所无法实现的。本综述介绍了RNA测序技术及其如何为肌肉骨骼再生医学的进展铺平道路。
再生医学领域的近期研究利用RNA测序来解读病理生理机制，并确定再生医学的新靶点。该技术还通过对干细胞的深入表征、确定分化轨迹和优化细胞培养条件，推动了干细胞生物学的发展。它还提供了新知识，从而改进了肌肉骨骼再生医学中生长因子的使用和支架设计。本文综述了肌肉骨骼再生医学领域近期利用RNA测序的研究。它展示了转录组分析如何能够用于提供有助于制定再生策略的见解。
