Ignatova Milena, Manolova Nevena, Rashkov Iliya, Georgieva Ani, Toshkova Reneta, Markova Nadya
Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 103A, BG-1113 Sofia, Bulgaria.
Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 25, BG-1113 Sofia, Bulgaria.
Polymers (Basel). 2023 Jul 24;15(14):3140. doi: 10.3390/polym15143140.
Novel fibrous materials with diverse biological properties containing a model drug of the 8-hydroxyquinoline group-5-amino-8-hydroxyquinoline (5A8Q)-were fabricated using a one-pot method by electrospinning poly(vinyl alcohol) (PVA)/carboxymethyl cellulose (CMC)/5A8Q solutions. Experiments were performed to prepare Cu (Fe) complexes of the crosslinked PVA/CMC/5A8Q materials. The formation of complexes was proven by using scanning electron microscopy (SEM), attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy, energy-dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS). The release of 5A8Q and 5A8Q.Cu (Fe) was studied and their in vitro release profiles were mostly impacted by the hydrophilic/hydrophobic properties of the materials. The performed microbiological assays revealed that fibrous materials containing 5A8Q and their complexes exhibited good antibacterial and antifungal efficacy. Their activity was stronger against bacteria than against bacteria and fungi . Cell viability tests using MTT showed that the presence of 5A8Q and its complexes in the fibrous materials resulted in a significant decrease in the HeLa and MCF-7 cancer cell viability for the various times of cell incubation. Moreover, the observed cytotoxicity of the mats against cancer cells was greater than that against non-cancer HaCaT keratinocytes. All these properties make the novel materials potential candidates for the design of wound healing materials and as drug delivery systems for local therapy of cervical and breast cancer.
采用一锅法通过静电纺丝制备含8-羟基喹啉类模型药物5-氨基-8-羟基喹啉（5A8Q）的具有多种生物学特性的新型纤维材料，该材料由聚乙烯醇（PVA）/羧甲基纤维素（CMC）/5A8Q溶液制成。进行实验制备交联PVA/CMC/5A8Q材料的铜（铁）配合物。通过扫描电子显微镜（SEM）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）、能量色散X射线光谱（EDX）和X射线光电子能谱（XPS）证实了配合物的形成。研究了5A8Q和5A8Q·Cu（Fe）的释放情况，其体外释放曲线主要受材料亲水性/疏水性的影响。所进行的微生物学检测表明，含5A8Q的纤维材料及其配合物具有良好的抗菌和抗真菌功效。它们对细菌的活性比对细菌和真菌的活性更强。使用MTT进行的细胞活力测试表明，纤维材料中5A8Q及其配合物的存在导致HeLa和MCF-7癌细胞在不同细胞培养时间的活力显著下降。此外，观察到的材料对癌细胞的细胞毒性大于对非癌性HaCaT角质形成细胞的细胞毒性。所有这些特性使这些新型材料成为伤口愈合材料设计以及作为宫颈癌和乳腺癌局部治疗药物递送系统的潜在候选材料。
