一种用于单细胞全旋转软 X 射线断层摄影术的协议。
A protocol for full-rotation soft X-ray tomography of single cells.
机构信息
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA.
出版信息
STAR Protoc. 2022 Feb 10;3(1):101176. doi: 10.1016/j.xpro.2022.101176. eCollection 2022 Mar 18.
The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021).
该方案描述了使用软 X 射线断层扫描技术逐步进行样本制备、数据获取和细胞细胞器分割的过程。它是专为在圆柱形样本支架中对样本进行全旋转数据采集的显微镜而设计的，例如劳伦斯伯克利国家实验室先进光源上的 XM-2 显微镜；然而，它也可以推广到类似的用于同步辐射和台式显微镜的样本支架设计。如需了解该方案的使用和执行的详细信息，请参考 Loconte 等人（2021 年）的文献。
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