Department of Biomaterials, The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, College of Materials, Xiamen University, Xiamen 361005, People's Republic of China.
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, United States.
ACS Appl Mater Interfaces. 2022 Mar 9;14(9):11104-11115. doi: 10.1021/acsami.1c24231. Epub 2022 Feb 24.
Nanozymes with peroxidase-like activity have great application potential in combating pathogenic bacterial infections and are expected to become an alternative to antibiotics. However, the near-neutral pH and high glutathione (GSH) levels in the bacterial infection microenvironment severely limit their applications in antibacterial therapy. In this work, a metal-organic framework (MOF)-based cascade catalytic glutathione-depleting system named MnFeO@MIL/Au&GOx (MMAG) was constructed. The MMAG cascade-catalyzed glucose to provide H and produces a large amount of toxic reactive oxygen species. In addition, MMAG consumed GSH, which can result in bacterial death more easily. Systematic antibacterial experiments illustrated that MMAG has superior antibacterial effects on both Gram-positive bacteria and Gram-negative bacteria.
具有过氧化物酶样活性的纳米酶在对抗致病性细菌感染方面具有巨大的应用潜力，有望成为抗生素的替代品。然而，细菌感染微环境中的近中性 pH 值和高谷胱甘肽 (GSH) 水平严重限制了它们在抗菌治疗中的应用。在这项工作中，构建了一种基于金属有机骨架 (MOF) 的级联催化谷胱甘肽耗竭系统，命名为 MnFeO@MIL/Au&GOx (MMAG)。MMAG 级联催化葡萄糖提供 H+并产生大量毒性活性氧。此外，MMAG 消耗 GSH，这使得细菌更容易死亡。系统的抗菌实验表明，MMAG 对革兰氏阳性菌和革兰氏阴性菌均具有优异的抗菌效果。
