Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS, 31300 Toulouse, France.
Toxins (Basel). 2022 Jan 22;14(2):83. doi: 10.3390/toxins14020083.
Fumonisin B1 (FB1) is a widespread mycotoxin produced by fungal species-mainly in maize, one of the plants most commonly used for food and feed. Pigs and horses are the animal species most susceptible to this mycotoxin. FB1 exposure can cause highly diverse clinical symptoms, including hepatotoxicity, immunotoxicity, and intestinal barrier function disturbance. Inhibition of ceramide synthetase is a well-understood ubiquitous molecular mechanism of FB1 toxicity, but other more tissue-specific effects remain to be elucidated. To investigate the effects of FB1 in different exposed tissues, we cross-analyzed the transcriptomes of fours organs: liver, jejunum, jejunal Peyer's patches, and spleen. During a four-week study period, pigs were fed a control diet or a FB1-contaminated diet (10 mg/kg feed). In response to oral FB1 exposure, we observed common biological processes in the four organs, including predominant and recurrent processes (extracellular matrix organization, integrin activation, granulocyte chemotaxis, neutrophil migration, and lipid and sterol homeostasis), as well as more tissue-specific processes that appeared to be related to lipid outcomes (cell cycle regulation in jejunum, and gluconeogenesis in liver).
伏马菌素 B1（FB1）是一种广泛存在的真菌毒素，主要由真菌产生，常见于玉米等植物中，这些植物是最常被用于食品和饲料的原料之一。猪和马是最易受这种真菌毒素影响的动物物种。FB1 暴露会引起高度多样化的临床症状，包括肝毒性、免疫毒性和肠道屏障功能障碍。鞘氨醇合酶的抑制是 FB1 毒性的一种众所周知的普遍分子机制，但其他更具组织特异性的作用仍有待阐明。为了研究 FB1 在不同暴露组织中的作用，我们对四个器官（肝脏、空肠、空肠派尔集合淋巴结和脾脏）的转录组进行了交叉分析。在为期四周的研究期间，猪被喂食对照饮食或 FB1 污染饮食（饲料中 10mg/kg）。在口服 FB1 暴露后，我们观察到四个器官中存在共同的生物学过程，包括主要和反复出现的过程（细胞外基质组织、整合素激活、粒细胞趋化、中性粒细胞迁移以及脂质和固醇稳态），以及更具组织特异性的过程，这些过程似乎与脂质结局有关（空肠中的细胞周期调节和肝脏中的糖异生）。
