Selim Samy, Faried Osama Ahmed, Almuhayawi Mohamed S, Mohammed Osama A, Saleh Fayez M, Warrad Mona
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72341, Saudi Arabia.
Medical Microbiology and Immunology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef 62513, Egypt.
Antibiotics (Basel). 2022 Jan 28;11(2):168. doi: 10.3390/antibiotics11020168.
(), a nonfermenting Gram-negative bacterium, has recently been associated with a broad range of nosocomial infections. To gain more meaningful insight into the problem of nosocomial illnesses caused by the multidrug-resistant (MDR) , as well as the factors that increase the risk of catching these infections, this investigation included a total of 86 clinical infections. Repetitive extragenic palindromic (REP)-PCR was used to investigate imipenem-resistant isolates for dynamic gene clusters causing carbapenem resistance. Four distinct lineages were found in the REP-PCR-DNA fingerprints of all isolates, with 95% of the samples coming from two dominant lineages. Imipenem, amikacin, and ciprofloxacin were less effective against genotype (A) isolates because of enhanced antibiotic tolerance. Lastly, to gain more insight into the mode of action of imipenem, we explored the binding affinity of imipenem toward different OXA beta-lactamase class enzymes.
（某菌名），一种非发酵革兰氏阴性菌，最近与多种医院感染相关。为了更深入了解由多重耐药（MDR）（某菌名）引起的医院感染问题以及增加感染这些病菌风险的因素，本研究共纳入了86例临床感染病例。采用重复外源性回文序列（REP）-PCR技术研究耐亚胺培南菌株中导致碳青霉烯耐药的动态基因簇。在所有分离株的REP-PCR-DNA指纹图谱中发现了四个不同的谱系，95%的样本来自两个主要谱系。由于抗生素耐受性增强，亚胺培南、阿米卡星和环丙沙星对基因型（A）分离株的疗效较差。最后，为了更深入了解亚胺培南的作用模式，我们探究了亚胺培南对不同OXAβ-内酰胺酶类酶的结合亲和力。
