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苦荞中DNA结合单指(DOF)基因家族的全基因组鉴定及其外源激素刺激后的表达模式分析

Genome-Wide Identification of DNA Binding with One Finger () Gene Family in Tartary Buckwheat () and Analysis of Its Expression Pattern after Exogenous Hormone Stimulation.

作者信息

Li Jing, Zhang Yuchuan, Xu Lei, Wang Chenyang, Luo Yan, Feng Shan, Yuan Yuhao, Yang Qinghua, Feng Baili

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712000, China.

School of Mathematics and Statistics, Northwestern Polytechnical University, Xi'an 710129, China.

出版信息

Biology (Basel). 2022 Jan 21;11(2):173. doi: 10.3390/biology11020173.

Abstract

DNA binding with one finger () proteins have been proven to be involved in multiple biological processes. However, genome-wide identification of the gene family has not been reported for Tartary buckwheat (). In this study, 35 Dof proteins were identified, and they could be divided into nine phylogenetic subgroups. Proteins within the same subgroup had similar gene structure and motif composition. Moreover, abundant -acting elements were present in the promoter regions of genes. Segmental duplication was the primary driving force for the evolution of the Dof gene family. Synteny analysis indicated that Tartary buckwheat was closer to dicotyledons, and more orthologous genes existed among them. The expression pattern of in different tissues and at different fruit developmental stages varied. Different tissues contained several genes that were specifically expressed. expression was mainly upregulated under methyl jasmonate treatment and downregulated under other hormone treatments. Taken together, may play important roles in the growth and development of Tartary buckwheat and in response to abiotic and biotic stresses. Therefore, the genome-wide identification and expression pattern analysis of the Tartary buckwheat gene family lays a foundation for further exploration of the functional characteristics of in the future.

摘要

单指DNA结合(Dof)蛋白已被证明参与多种生物学过程。然而,苦荞的Dof基因家族全基因组鉴定尚未见报道。本研究鉴定出35个Dof蛋白,它们可分为9个系统发育亚组。同一亚组内的蛋白具有相似的基因结构和基序组成。此外,Dof基因的启动子区域存在丰富的顺式作用元件。片段重复是Dof基因家族进化的主要驱动力。共线性分析表明,苦荞与双子叶植物的亲缘关系更近,它们之间存在更多的直系同源Dof基因。Dof在不同组织和不同果实发育阶段的表达模式有所不同。不同组织中含有几个特异性表达的基因。Dof表达在茉莉酸甲酯处理下主要上调,在其他激素处理下下调。综上所述,Dof可能在苦荞的生长发育以及对非生物和生物胁迫的响应中发挥重要作用。因此,苦荞Dof基因家族的全基因组鉴定和表达模式分析为未来进一步探索Dof的功能特性奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dd1/8869700/4f58ea628ff9/biology-11-00173-g001.jpg

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