Xiao Xuefeng, Chen Jingzhong, Liao Xiaofeng, Yan Qiuxiao, Liang Gelin, Liu Jiming, Wang Deng, Guan Ruiting
Forestry College, Research Center of Forest Ecology, Guizhou University, Guiyang 550025, China.
Institute of Mountain Resources, Guizhou Academy of Science, Guiyang 550001, China.
Biology (Basel). 2022 Jan 29;11(2):220. doi: 10.3390/biology11020220.
Drought stress is one of the greatest obstacles affecting field crop productivity in arid and semi-arid regions, and its severity and frequency are expected to increase due to human-induced changes to the environment and climate. Drought has led to rocky desertification in Karst regions. is a unique, fast-growing medicinal plant of Southwest China that only thrives in Karst regions. Arbuscular mycorrhizal fungi (AMF) symbiosis alleviates drought stress in plants; however, establishment and function of the symbiotic interaction between AMF host plant in relation to the inoculation method remain unclear. Therefore, we conducted an experiment to investigate the effects of AMF species ( and ) and two inoculation methods (seed vs. seedling inoculation) under drought stress on seedlings, and quantified mycorrhizal colonization, AMF spore density, root vigor, relative water content, growth, antioxidant enzyme activities, and osmotic adjustment. Inoculation with AMF ( and ) positively affected the growth and root vigor of under drought stress, regardless of the inoculation method. Additionally, both AMF species markedly upregulated antioxidant enzyme activities and osmotic adjustment substances, regardless of the inoculation method. Our results showed that the collective stimulatory effect of is more efficient than that of . AMF application could promote afforestation with to prevent rocky desertification in Karst regions where water is the greatest limiting factor on plant growth and yield.
干旱胁迫是影响干旱和半干旱地区大田作物生产力的最大障碍之一，由于人为引起的环境和气候变化，其严重程度和发生频率预计将会增加。干旱已导致喀斯特地区石漠化。[植物名称]是中国西南部一种独特的、生长迅速的药用植物，仅在喀斯特地区生长旺盛。丛枝菌根真菌（AMF）共生可缓解植物的干旱胁迫；然而，关于接种方法，AMF与宿主植物之间共生相互作用的建立和功能仍不清楚。因此，我们进行了一项实验，以研究干旱胁迫下AMF物种（[物种名称1]和[物种名称2]）和两种接种方法（种子接种与幼苗接种）对[植物名称]幼苗的影响，并对菌根定殖、AMF孢子密度、根系活力、相对含水量、[植物名称]生长、抗氧化酶活性和渗透调节进行了量化。无论接种方法如何，接种AMF（[物种名称1]和[物种名称2]）均对干旱胁迫下的[植物名称]生长和根系活力产生积极影响。此外，无论接种方法如何，两种AMF物种均显著上调抗氧化酶活性和渗透调节物质。我们的结果表明，[物种名称1]的总体刺激作用比[物种名称2]更有效。在水分是植物生长和产量最大限制因素的喀斯特地区，应用AMF可以促进[植物名称]造林以防止石漠化。
