Chen Xiaodan, Li Wei, Chen Jianying, Zhang Xiaoyong, Zhang Wei, Duan Xuewu, Lei Bingfu, Huang Riming
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
J Fungi (Basel). 2022 Apr 20;8(5):420. doi: 10.3390/jof8050420.
(), a citrus blue mold, is a pathogenic fungus that greatly affects the postharvest quality of citrus fruits with significant economic loss. Our previous research showed that 2-methoxy-1, 4-naphthoquinone (MNQ) inhibited the growth of . However, the water dispersibility of MNQ will limit its further application. Herein, we synthesized MNQ-based carbon dots (2-CDs) with better water dispersibility, which showed a potential inhibitory effect on (MIC = 2.8 μg/mL) better than that of MNQ (MIC = 5.0 μg/mL). Transcriptomics integrated with metabolomics reveals a total of 601 differentially enriched genes and 270 differentially accumulated metabolites that are co-mapped as disruptive activity on the cell cytoskeleton, glycolysis, and histone methylation. Furthermore, transmission electron microscopy analysis showed normal appearances and intracellular septum of after treatment. These findings contribute tofurther understanding of the possible molecular action of 2-CDs.
柑橘青霉（Penicillium digitatum）是一种致病性真菌，会严重影响柑橘类水果的采后品质，造成重大经济损失。我们之前的研究表明，2-甲氧基-1,4-萘醌（MNQ）能抑制柑橘青霉的生长。然而，MNQ的水分散性会限制其进一步应用。在此，我们合成了具有更好水分散性的基于MNQ的碳点（2-CDs），其对柑橘青霉显示出潜在抑制作用（MIC = 2.8 μg/mL），优于MNQ（MIC = 5.0 μg/mL）。转录组学与代谢组学相结合揭示了总共601个差异富集基因和270个差异积累代谢物，它们共同映射到对细胞细胞骨架、糖酵解和组蛋白甲基化的破坏活性上。此外，透射电子显微镜分析显示处理后的柑橘青霉外观正常且有细胞内隔膜。这些发现有助于进一步了解2-CDs可能的分子作用。
