Hashem Amr H, Al-Askar Abdulaziz A, Haponiuk Józef, Abd-Elsalam Kamel A, Hasanin Mohamed S
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
Department of Botany and Microbiology, Faculty of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Microorganisms. 2023 May 24;11(6):1380. doi: 10.3390/microorganisms11061380.
Metal nanoparticles are assumed to be a new generation of biologically active materials. The integrations between more than one metal are synergetic multifunctional features. In the current study, trimetallic copper-selenium-zinc oxide nanoparticles (Tri-CSZ NPs) were successfully mycosynthesized using through an ecofriendly method for the first time. The biosynthesis of the particles was characterized using physiochemical and topographical analysis. The physiochemical analysis included Fourier transform infrared spectroscopy (FTIR), which affirmed that the biosynthesis of Tri-CSZ NPs relies on the functional groups of fungal filtrates. Additionally, the UV-visible and X-ray diffraction patterns were proposed for the formation of Tri-CSZ NPs; moreover, topography analysis confirmed that the micromorphology of the nanoparticles were similar to a stick, with ends having a tetragonal pyramid shape, and with an average nanosize of about 26.3 ± 5.4 nm. Cytotoxicity results reveled that the Tri-CSZ NPs have no cytotoxicity on the human normal cell line Wi 38 at low concentrations, where the IC50 was 521 µg/mL. Furthermore, the antifungal activity of the Tri-CSZ NPs was evaluated. The antifungal results revealed that the Tri-CSZ NPs have promising antifungal activity against , , , and , where the minimum inhibitory concentrations (MICs) were 1.95, 7.81, 62.5, and 3.9 µg/mL, and the minimum fungicidal concentrations (MFCs) were 250, 62.5, 125, and 1000 µg/mL, respectively. In conclusion, Tri-CSZ NPs were successfully mycosynthesized using , which have a promising antifungal activity against fungi causing mucormycosis.
金属纳米颗粒被认为是新一代生物活性材料。多种金属之间的整合具有协同多功能特性。在当前研究中，首次通过一种环保方法成功地利用真菌合成了三金属铜 - 硒 - 氧化锌纳米颗粒（Tri - CSZ NPs）。使用物理化学和形貌分析对颗粒的生物合成进行了表征。物理化学分析包括傅里叶变换红外光谱（FTIR），其证实Tri - CSZ NPs的生物合成依赖于真菌滤液的官能团。此外，还给出了Tri - CSZ NPs形成的紫外 - 可见光谱和X射线衍射图谱；而且，形貌分析证实纳米颗粒的微观形态类似于棒状，两端为四方金字塔形状，平均纳米尺寸约为26.3±5.4 nm。细胞毒性结果表明，在低浓度下，Tri - CSZ NPs对人正常细胞系Wi 38没有细胞毒性，其半数抑制浓度（IC50）为521μg/mL。此外，还评估了Tri - CSZ NPs的抗真菌活性。抗真菌结果表明，Tri - CSZ NPs对米根霉、根霉、毛霉和烟曲霉具有良好的抗真菌活性，其最低抑菌浓度（MICs）分别为1.95、7.81、62.5和3.9μg/mL，最低杀菌浓度（MFCs）分别为250、62.5、125和1000μg/mL。总之，利用真菌成功合成了Tri - CSZ NPs，其对引起毛霉病的真菌具有良好的抗真菌活性。
