Wang Ya-Wen, Chen Xu, Tian Yaru, Liu Long, Su Peng
Department of Breast Surgery, General Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Department of Pathology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Front Mol Biosci. 2022 Feb 8;8:690826. doi: 10.3389/fmolb.2021.690826. eCollection 2021.
Circular RNAs (circRNAs) have been shown to play important roles in the development and progression of human cancers. Emerging evidence shows that circRNAs have the potential to be promising biomarkers for cancer diagnosis and prognosis. However, the roles of circRNAs in breast cancer axillary lymph node metastasis (ALNM) remain to be determined. Transcriptome sequencing was utilized to screen the differentially expressed circRNAs in three breast cancer tissues with ALNM and three without ALNM. Differentially expressed circRNAs were further verified by quantitative real-time PCR. Moreover, receiver operating characteristic (ROC) curve analysis was performed to calculate the value of circRNAs to distinguish breast cancer tissues with ALNM and those without ALNM. To explore the potential mechanism of the circRNAs, a circRNA-miRNA-mRNA network was constructed based on the CircInteractome, circBank, and mirDIP online software. In total, 31 differentially expressed circRNAs were identified by transcriptome sequencing; among them, 21 were upregulated and 10 were downregulated in breast cancer with ALNM compared to those without ALNM. Circ_0000160 was validated to be downregulated in breast cancer tissues with ALNM compared with those without ALNM. The ROC curve showed the ability of circ_0000160 to distinguish breast cancer tissues with ALNM and those without ALNM, with an area under the curve of 0.7435. Furthermore, bioinformatics analysis revealed that the predicted mRNAs for circ_0000160 may be related to lymph node metastasis. The predicted mRNAs for circ_0000160 may be involved in many cancer-related pathways. A decreased expression of circ_0000160 was found in breast cancer with axillary lymph node metastasis. Circ_0000160 may have the potential to distinguish breast cancer with axillary lymph node metastasis from those without axillary lymph node metastasis.
环状RNA（circRNAs）已被证明在人类癌症的发生和发展中发挥重要作用。新出现的证据表明，circRNAs有潜力成为癌症诊断和预后的有前景的生物标志物。然而，circRNAs在乳腺癌腋窝淋巴结转移（ALNM）中的作用仍有待确定。利用转录组测序筛选了3个有ALNM的乳腺癌组织和3个无ALNM的乳腺癌组织中差异表达的circRNAs。通过定量实时PCR进一步验证差异表达的circRNAs。此外，进行了受试者工作特征（ROC）曲线分析，以计算circRNAs区分有ALNM和无ALNM的乳腺癌组织的价值。为了探索circRNAs的潜在机制，基于CircInteractome、circBank和mirDIP在线软件构建了circRNA- miRNA- mRNA网络。通过转录组测序共鉴定出31个差异表达的circRNAs；其中，与无ALNM的乳腺癌相比，有ALNM的乳腺癌中有21个上调，10个下调。与无ALNM的乳腺癌组织相比，Circ_0000160在有ALNM的乳腺癌组织中被验证为下调。ROC曲线显示Circ_0000160区分有ALNM和无ALNM的乳腺癌组织的能力，曲线下面积为0.7435。此外，生物信息学分析表明，Circ_0000160的预测mRNA可能与淋巴结转移有关。Circ_0000160的预测mRNA可能参与许多癌症相关途径。在有腋窝淋巴结转移的乳腺癌中发现Circ_0000160表达降低。Circ_0000160可能有潜力区分有腋窝淋巴结转移和无腋窝淋巴结转移的乳腺癌。
