Amine-Khodja Ihsein Rokia, Boscari Alexandre, Riah Nassira, Kechid Maya, Maougal Rim Tinhinen, Belbekri Nadir, Djekoun Abdelhamid
Genetic, Biochemistry and Plant Biotechnology Laboratory, Faculty of Natural Sciences, Constantine 1 University, Route Ain El Bey, Constantine 25000, Algeria.
INRAE, Université Côte d'Azur, CNRS, ISA, 06903 Sophia Antipolis, France.
Plants (Basel). 2022 Feb 14;11(4):515. doi: 10.3390/plants11040515.
Drought stress has become one of the most uncontrolled and unpredictable constraints on crop production. The purpose of this study was to evaluate the impacts of two different strains on terminal drought tolerance induction in two faba bean genotypes cultivated in Algeria, Aquadulce and Maltais. To this end, we measured physiological parameters-osmoprotectants accumulation, oxidative stress markers and enzyme activities-to assess the effect of inoculation on under terminal water deficiency conditions in greenhouse trials. Upregulation of anti-oxidative mechanisms and production of compatible solutes were found differentially activated according to strain. Drought stress resilience of the Maltais variety was improved using the local strain OL13 compared to the common strain 3841. Symbiosis with OL13 strain leads in particular to a much better production of proline and soluble sugar in nodules but also in roots and leaves of Maltais plant. Even if additional work is still necessary to decipher the mechanism by which a strain can affect the accumulation of osmoprotectants or cellular redox status in all the plants, inoculation with selected could be a promising strategy for improving water stress management in the forthcoming era of climate change.
干旱胁迫已成为作物生产中最难以控制和预测的限制因素之一。本研究的目的是评估两种不同菌株对在阿尔及利亚种植的两种蚕豆基因型（阿夸杜尔塞和马尔泰斯）终端干旱耐受性诱导的影响。为此，我们测量了生理参数——渗透保护剂积累、氧化应激标志物和酶活性——以评估接种在温室试验中对终端水分亏缺条件下的影响。根据菌株的不同，发现抗氧化机制的上调和相容性溶质的产生被差异激活。与普通菌株3841相比，使用当地菌株OL13提高了马尔泰斯品种的干旱胁迫恢复力。与OL13菌株共生尤其导致马尔泰斯植物的根瘤、根和叶中脯氨酸和可溶性糖的产量显著提高。即使仍需要额外的工作来破译一种菌株影响所有植物中渗透保护剂积累或细胞氧化还原状态的机制，但接种选定的菌株可能是在即将到来的气候变化时代改善水分胁迫管理的一种有前景的策略。
