Kang Yanan, Nack Leroy Marwin, Liu Yang, Qi Bing, Huang Yalan, Liu Ziyao, Chakraborty Indranath, Schulz Florian, Ahmed Abdullah A A, Clavo Poveda Mirco, Hafizi Fereshta, Roy Sathi, Mutas Marina, Holzapfel Malte, Sanchez-Cano Carlos, Wegner K David, Feliu Neus, Parak Wolfgang J
Center for Hybrid Nanostructures (CHyN) and Fachbereich Physik and Chemie, Universität Hamburg, Hamburg, Germany.
Present Address: Key Laboratory of Biological Nanotechnology of National Health Commission, Xiangya Hospital, Central South University, Changsha, Hunan China.
ChemTexts. 2022;8(1):9. doi: 10.1007/s40828-021-00159-6. Epub 2022 Jan 25.
Most studies about the interaction of nanoparticles (NPs) with cells have focused on how the physicochemical properties of NPs will influence their uptake by cells. However, much less is known about their potential excretion from cells. However, to control and manipulate the number of NPs in a cell, both cellular uptake and excretion must be studied quantitatively. Monitoring the intracellular and extracellular amount of NPs over time (after residual noninternalized NPs have been removed) enables one to disentangle the influences of cell proliferation and exocytosis, the major pathways for the reduction of NPs per cell. Proliferation depends on the type of cells, while exocytosis depends in addition on properties of the NPs, such as their size. Examples are given herein on the role of these two different processes for different cells and NPs.
大多数关于纳米颗粒（NPs）与细胞相互作用的研究都集中在NPs的物理化学性质如何影响细胞对它们的摄取。然而，关于它们从细胞中潜在排泄的情况却知之甚少。然而，为了控制和操纵细胞内NPs的数量，必须对细胞摄取和排泄进行定量研究。随着时间的推移监测细胞内和细胞外NPs的量（在去除残留的未内化NPs之后），能够使人们区分细胞增殖和胞吐作用的影响，这是每个细胞中NPs减少的主要途径。增殖取决于细胞类型，而胞吐作用还取决于NPs的性质，例如它们的大小。本文给出了这两个不同过程对不同细胞和NPs作用的例子。
