Barsomian G D, Robillard N J, Thorne C B
J Bacteriol. 1984 Mar;157(3):746-50. doi: 10.1128/jb.157.3.746-750.1984.
Three groups of linked markers were mapped in Bacillus thuringiensis 4042B by using two-, three-, and four-factor crosses mediated by the temperate bacteriophages TP-13 and TP-18. The order of markers was (trp-11, trp-2)-(leu-1, leu-2)-his-1-(lys-1, lys-2)-cys-1 in the first group; met-1-(argCl, argOl)-met-2-(pyr-1, pyrA2) in the second group; and met-3-pur-1-(nal-1, nal-2)-str-1-(pur-2, pur-4)-pur-3 in the third group. Electron microscopic measurements of head sizes suggested that the volume of the TP-13 phage head is seven times greater than that of the TP-18 phage head. The TP-18 genome was shown by DNA restriction analysis to have a molecular mass of 36 megadaltons. TP-13 was useful for scanning large segments of the B. thuringiensis chromosome, and TP-18 was effective for ordering markers too closely linked for simple resolution with TP-13.
利用温和噬菌体TP - 13和TP - 18介导的双因子、三因子和四因子杂交，在苏云金芽孢杆菌4042B中定位了三组连锁标记。第一组标记的顺序为(trp - 11, trp - 2)-(leu - 1, leu - 2)-his - 1-(lys - 1, lys - 2)-cys - 1；第二组为met - 1-(argCl, argOl)-met - 2-(pyr - 1, pyrA2)；第三组为met - 3-pur - 1-(nal - 1, nal - 2)-str - 1-(pur - 2, pur - 4)-pur - 3。对噬菌体头部大小的电子显微镜测量表明，TP - 13噬菌体头部的体积比TP - 18噬菌体头部的体积大七倍。通过DNA限制性分析表明，TP - 18基因组的分子量为36兆道尔顿。TP - 13可用于扫描苏云金芽孢杆菌染色体的大片段，而TP - 18对于排列那些与TP - 13简单分辨时连锁过紧密的标记有效。
