Aksoy Laçine, Güzey İsmail, Düz Mürüvvet
Afyon Kocatepe University, Faculty of Science and Arts, Department of Chemistry, Afyonkarahisar, Turkey
Turk J Pharm Sci. 2022 Feb 28;19(1):76-83. doi: 10.4274/tjps.galenos.2021.86422.
Our research measures the essential oil analysis mineral substance profiles, total phenolic substance content, free radical scavenger properties, antioxidant capacity, and enzyme inhibitory activity of Aytaç, Ekici & Dönmez, 1996.
A mixture of plant roots and stems were used. Essential fatty acid components of were determined by gas chromatography-mass spectrometry and bioelement concentrations by inductively coupled plasma optical emission spectrometry. The antioxidant activity of extracts was screened by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, total phenolic content, total antioxidant status (TAS), total oxidant status (TOS) analysis. Cholinesterase (ChE), α-glucosidase, α-amylase, and tyrosinase inhibitory activity was determined.
The results demonstrated that the phenolic substance content was higher in methanol extract (144.08±2.01 μg gallic acid equivalent/mg extract). DPPH scavenging effect of methanol extract (73.2%) was higher than acetone extract (60.1%). TAS values of extract methanol and acetone were 2.32±0.4 and 2.38±0.2 μmol trolox Eq /g, TOS values were 4.88±0.6 and 5.04±0.5 μmol HO Eq/g, and oxidative stress index values were 2.1±0.3 and 2.11±0.24 arbitrary units, respectively. Hexadecanoic acid (17.9%) was found as the main component in the plant essential oil. species was prominent with high Mg and Al concentrations. Anti-ChE activity was determined that acetone extract (42.95%±0.90; 217.37±0.81 mg galantamine equivalents [(GALAEs)/g] exhibited higher than methanol (33.33%±1.81; 208.76±1.62 mg GALAE/g). α-Amylase inhibition was high in methanol extract [53.62±1.85 mmol angiotensin converting enzymes (ACEs)/g extract] compared to acetone (47.73±0.92 mmol ACEs/g extract). The tyrosinase inhibitory activity of S. akmanii was determined very low inhibition of the reference compound.
It has been determined that Aytaç, Ekici & Dönmez extracts have antioxidant properties and can inhibit acetylcholinesterase, α-glucosidase, α-amylase enzymes. This study is informative on future studies on a highly bioavailable species and very extensive studies should be carried out.
我们的研究测量了1996年Aytaç、Ekici和Dönmez的精油分析矿物质概况、总酚类物质含量、自由基清除特性、抗氧化能力和酶抑制活性。
使用植物根和茎的混合物。通过气相色谱-质谱法测定其必需脂肪酸成分，通过电感耦合等离子体发射光谱法测定生物元素浓度。通过2,2-二苯基-1-苦基肼（DPPH）自由基清除活性、总酚含量、总抗氧化状态（TAS）、总氧化状态（TOS）分析来筛选提取物的抗氧化活性。测定胆碱酯酶（ChE）、α-葡萄糖苷酶、α-淀粉酶和酪氨酸酶的抑制活性。
结果表明，甲醇提取物中的酚类物质含量较高（144.08±2.01μg没食子酸当量/毫克提取物）。甲醇提取物的DPPH清除效果（73.2%）高于丙酮提取物（60.1%）。提取物甲醇和丙酮的TAS值分别为2.32±0.4和2.38±0.2μmol Trolox当量/克，TOS值分别为4.88±0.6和5.04±0.5μmol HO当量/克，氧化应激指数值分别为2.1±0.3和2.11±0.24任意单位。十六烷酸（17.9%）被发现是植物精油中的主要成分。该物种以高镁和铝浓度突出。测定抗ChE活性时，发现丙酮提取物（42.95%±0.90；217.37±0.81毫克加兰他敏当量[(GALAEs)/克]）高于甲醇提取物（33.33%±1.81；208.76±1.62毫克GALAE/克）。与丙酮提取物（47.73±0.92毫摩尔ACE/克提取物）相比，甲醇提取物中的α-淀粉酶抑制率较高[53.62±1.85毫摩尔血管紧张素转换酶（ACE）/克提取物]。测定了Akmanii链霉菌的酪氨酸酶抑制活性，发现其对参考化合物的抑制作用非常低。
已确定Aytaç、Ekici和Dönmez提取物具有抗氧化特性，并且可以抑制乙酰胆碱酯酶、α-葡萄糖苷酶、α-淀粉酶。这项研究为未来对这种具有高生物利用度的物种的研究提供了信息，应该进行非常广泛的研究。
