Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, The Netherlands.
CEA, CNRS, Institut de Biologie Structurale (IBS), Université Grenoble Alpes, France.
FEBS Lett. 2022 Apr;596(7):958-969. doi: 10.1002/1873-3468.14324. Epub 2022 Mar 9.
The Cdv proteins constitute the cell division system of the Crenarchaea, a machinery closely related to the ESCRT system of eukaryotes. Using a combination of TEM imaging and biochemical assays, we here present an in vitro study of Metallosphaera sedula CdvB1, the Cdv protein that is believed to play a major role in the constricting ring that drives cell division in the Crenarchaea. We show that CdvB1 self-assembles into filaments that are depolymerized by the Vps4-homolog ATPase CdvC. Furthermore, we find that CdvB1 binds to negatively charged lipid membranes and can be detached from the membrane by the action of CdvC. Our findings provide novel insight into one of the main components of the archaeal cell division machinery.
Cdv 蛋白构成了古菌的细胞分裂系统，这是一种与真核生物的 ESCRT 系统密切相关的机制。通过 TEM 成像和生化分析的组合，我们在这里对被认为在古菌中驱动细胞分裂的收缩环中起主要作用的 Cdv 蛋白 Metallosphaera sedula CdvB1 进行了体外研究。我们表明，CdvB1 自组装成纤维，然后被 Vps4 同源 ATP 酶 CdvC 解聚。此外，我们发现 CdvB1 与带负电荷的脂质膜结合，并可以通过 CdvC 的作用从膜上脱离。我们的发现为古菌细胞分裂机制的主要成分之一提供了新的见解。
