Dengu Fungai, Neri Flavia, Ogbemudia Etohan, Ebeling Georg, Knijff Laura, Rozenberg Kaithlyn, Dumbill Richard, Branchereau Julien, Friend Peter, Ploeg Rutger, Hunter James
Oxford Transplant Centre, Nuffield Department of Surgical Sciences, University of Oxford, Oxford, UK.
Kidney and Pancreas Transplant Unit, Department of Surgery, Oncology and Gastroenterology, Padua University Hospital, Padua, Italy.
Ann Transl Med. 2022 Jan;10(1):1. doi: 10.21037/atm-21-2494.
Advances in organ preservation, reconditioning and assessment have been driven by the increasing necessity to utilise organs from extended criteria donors, particularly donors after circulatory death. Research efforts in this area have aided translation of machine perfusion technology into clinical practice. Pigs are anatomically and physiologically similar to humans and are an excellent model. However, conducting large animal experimental research is challenging and typically limited by ethical and economic constraints. Here we describe a reproducible, cost-effective multi-organ abdominal procurement model of porcine organs from the slaughterhouse.
Domestic pigs are electrically stunned and exsanguinated following the standard abattoir process. Via a longitudinal midline incision, the thoracoabdominal viscera are removed by incising along the anterior vertebral plane. The abdominal organs are isolated, perfused and separated preserving their respective vasculature, allowing individual organ use for specific experiments.
The warm ischaemic time is kept between 15-30 minutes. Using this highly protocolized procurement technique we have procured 12 livers, 162 kidneys and 12 pancreata for research, the majority of which have been utilized for perfusion experiments.
We have described a reliable and reproducible procedure for abdominal multi-organ procurement from slaughterhouse pigs.
随着使用扩大标准供体器官（尤其是心脏死亡后供体器官）的需求日益增加，器官保存、修复和评估方面取得了进展。该领域的研究工作推动了机器灌注技术转化为临床实践。猪在解剖学和生理学上与人类相似，是一种优秀的模型。然而，进行大型动物实验研究具有挑战性，通常受到伦理和经济限制。在此，我们描述了一种可重复、经济高效的从屠宰场获取猪腹部多器官的模型。
按照标准屠宰流程对家猪进行电击昏和放血。通过沿前正中线纵向切口，沿着前椎平面切开，取出胸腹内脏。分离、灌注并分离腹部器官，保留其各自的脉管系统，以便单个器官用于特定实验。
热缺血时间保持在15至30分钟之间。使用这种高度规范化的获取技术，我们已获取12个肝脏、162个肾脏和12个胰腺用于研究，其中大部分已用于灌注实验。
我们描述了一种从屠宰场猪获取腹部多器官的可靠且可重复的方法。
