College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, PR China.
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, PR China.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005257.
The 16S rRNA gene sequences of MM-14 and L53 shared 100 % sequence similarity. This study aimed to clarify the taxonomic position of the two species. Whole-genome comparisons showed that MM-14 and L53 shared average nucleotide identity of 97.52 %, digital DNA-DNA hybridization of 75.30 % and average amino acid identity of 96.98 %. These values exceeded the threshold of bacterial species delineation. Furthermore, average amino acid identities of MM-14 and L53 in comparison with CC-PY-50 were 82.04 and 82.11 %, respectively. Phylogenetic analysis based on 16S rRNA gene and 120 bacterial conserved single-copy genes also supported that MM-14 and L53 belonged to the genus . Phenotypic and chemical taxonomic properties compared between MM-14 and L53 were nearly identical. Colonies of the two species on marine agar plates were orange-pigmented, circular and smooth. Flexirubin-type pigments were present in both L53 and MM-14. The major fatty acids composition of the two species consisted of iso-C G, iso-C, and iso-C 3-OH, similar to CC-PY-50. Based on priority, He . 2018 is a later heterotypic synonym of Park . 2018, and should be transferred to the genus as comb. nov.
MM-14 和 L53 的 16S rRNA 基因序列完全相同，相似度为 100%。本研究旨在阐明这两个物种的分类地位。全基因组比较显示，MM-14 和 L53 的平均核苷酸同一性为 97.52%，数字 DNA-DNA 杂交为 75.30%，平均氨基酸同一性为 96.98%。这些值超过了细菌种划分的阈值。此外，MM-14 和 L53 与 CC-PY-50 的平均氨基酸同一性分别为 82.04%和 82.11%。基于 16S rRNA 基因和 120 个细菌保守单拷贝基因的系统发育分析也支持 MM-14 和 L53 属于 属。MM-14 和 L53 之间的表型和化学分类特性几乎相同。两者在海洋琼脂平板上的菌落均为橙色、圆形和光滑。L53 和 MM-14 中均存在柔韧菌素型色素。两种菌的主要脂肪酸组成均由 iso-C G、iso-C 和 iso-C 3-OH 组成，与 CC-PY-50 相似。根据优先权，He. 2018 是 Park. 2018 的晚异名，应作为 comb. nov. 转移到 属。
