作为预处理剂用 NaOCl 处理后 P11-4 自组装肽和 HAP 悬浮液的黏附性和美白效果。

Adhesion and whitening efficacy of P11-4 self-assembling peptide and HAP suspension after using NaOCl as a pre-treatment agent.

机构信息

Department of Conservative Dentistry and Periodontology, University Hospital, Ludwig-Maximilians-University Munich, Goethestr. 70, 80336, Munich, Germany.

出版信息

BMC Oral Health. 2022 Mar 4;22(1):59. doi: 10.1186/s12903-022-02080-x.

DOI:10.1186/s12903-022-02080-x

PMID:35246089

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8895591/

Abstract

BACKGROUND

This study evaluated the adhesion and whitening efficacy of a mixture of hydroxyapatite and P11-4 self-assembling peptide (HAP-peptide) on bovine enamel after pre-treatment with low-concentrated sodium hypochlorite (NaOCl).

METHODS

Fifty-two caries-free bovine incisors were selected. 50 teeth were randomly allocated to five groups (n = 10). The first group was treated with a mixture of 6.25 wt% HAP and 5 ml P11-4 peptide, using NaOCl 3% as pre-treatment. Second, third and fourth groups were treated with 6.25 wt% HAP, 5 ml P11-4 peptide, and NaOCl 3%, respectively. In the fifth group, only water was applied (control group). The color of samples was measured using a spectrophotometer (USB4000-VIS-NIR-ES, Ostfildern, Germany). To evaluate color changes, ΔE values were statistically analyzed. Finally, adherence of HAP particles on two enamel surfaces with and without pre-treatment with NaOCl was analyzed with SEM.

RESULTS

It was observed that the ΔE of the HAP-peptide suspension after pre-treatment with NaOCl was significantly stronger than the control group. In contrast, the overall color changes of separate applications of HAP, peptide, and NaOCl did not differ notably from the control group. SEM observations confirmed that pre-treatment with NaOCl resulted in a more pronounced coverage of HAP on the enamel surface.

CONCLUSIONS

Pre-treatment with a low-concentrated NaOCl enhanced the adherence of the HAP layer on the enamel surface, resulting in a stronger whitening effect.

TRIAL REGISTRATION

The peptide-HAP suspension is effective in improving tooth whiteness.

摘要

背景

本研究评估了经低浓度次氯酸钠（NaOCl）预处理后，羟基磷灰石和 P11-4 自组装肽（HAP-肽）混合物在牛牙釉质上的黏附性和美白效果。

方法

选择 52 颗无龋牛切牙。50 颗牙齿被随机分配到五组（n=10）。第一组用 6.25wt% HAP 和 5ml P11-4 肽混合物处理，用 3% NaOCl 作为预处理。第二、第三和第四组分别用 6.25wt% HAP、5ml P11-4 肽和 3% NaOCl 处理。第五组仅用水处理（对照组）。使用分光光度计（USB4000-VIS-NIR-ES，Ostfildern，德国）测量样品的颜色。为了评估颜色变化，对 ΔE 值进行了统计学分析。最后，用 SEM 分析了 NaOCl 预处理前后 HAP 颗粒在两个牙釉质表面的黏附情况。