温和派系统和被动超声冲洗联合微创与常规器械技术对抗感染根管中粪肠球菌脂磷壁酸的疗效。

Efficacy of GentleWave System and Passive Ultrasonic Irrigation with Minimally Invasive and Conventional Instrumentation Technique against Enterococcus faecalis Lipoteichoic Acid in Infected Root Canals.

机构信息

Division of Endodontics, Department of Advanced Oral Sciences and Therapeutics, University of Maryland, School of Dentistry, Baltimore, Maryland.

Department of Advanced Oral Sciences and Therapeutics, University of Maryland, School of Dentistry, Baltimore, Maryland; Department of Restorative Dental Science, Imam Abdulrahman Bin Faisal University, College of Dentistry, Dammam, Saudi Arabia.

出版信息

J Endod. 2022 Jun;48(6):768-774. doi: 10.1016/j.joen.2022.01.021. Epub 2022 Mar 3.

DOI:10.1016/j.joen.2022.01.021

PMID:35247369

Abstract

INTRODUCTION

This study evaluated the effectiveness of the GentleWave System (GWS; Sonendo, Laguna Hills, CA) and passive ultrasonic irrigation (PUI) in removing Enterococcus faecalis lipoteichoic acid (LTA) from infected root canals with a minimally invasive technique (MIT) and the conventional instrumentation technique (CIT).

METHODS

Sixty extracted human maxillary first premolars were included. All teeth were dentin pretreated and inoculated with LTA prepared from E. faecalis. First, 12 teeth were cryogenically ground to investigate the viability of recovering intraradicular E. faecalis LTA. Afterward, 48 teeth were randomly divided into the following groups: GWS + MIT, GWS + CIT, PUI + MIT, and GWS + CIT (all n = 12). Teeth were instrumented with a Vortex Blue (Dentsply Sirona, Ballaigues, Switzerland) rotary file size 15/.04 for MIT and 35/.04 for CIT. Samples were collected before and after a root canal procedure with sterile/apyrogenic paper points and after cryogenically ground for intraradicular LTA analysis. LTA was quantified with an LTA enzyme-linked immunosorbent assay kit.

RESULTS

E. faecalis LTA was recovered from 100% of the samples (48/48) before the root canal procedure. GWS + MIT and GWS + CIT were the most effective protocols against E. faecalis LTA, with no difference between them (P > .05). PUI + CIT was more effective than PUI + MIT (P < .05) but less effective than GWS + MIT and GWS + CIT (P < .05). The GWS groups showed more root canals with undetected E. faecalis LTA after treatment than all groups tested.

CONCLUSIONS

GWS + MIT and GWS + CIT were the most effective protocols against E. faecalis LTA in infected root canals.

摘要

简介

本研究采用微创技术（MIT）和传统器械预备技术（CIT）评估 GentleWave 系统（GWS；Sonendo，加利福尼亚州拉古纳山）和被动超声冲洗（PUI）去除感染根管内粪肠球菌脂磷壁酸（LTA）的效果。

方法

纳入 60 颗上颌第一前磨牙。所有牙齿均进行牙本质预处理并接种粪肠球菌 LTA 制备物。首先，12 颗牙齿经冷冻研磨以研究回收根管内粪肠球菌 LTA 的可行性。之后，48 颗牙齿随机分为以下组：GWS+MIT、GWS+CIT、PUI+MIT 和 GWS+CIT（每组 n=12）。使用 Vortex Blue（登士柏西诺德，Ballaigues，瑞士）旋转锉 15/.04 号锉（MIT）和 35/.04 号锉（CIT）预备牙齿。使用无菌/无热原纸尖在根管预备前后和冷冻研磨后收集根管内样本进行 LTA 分析。采用 LTA 酶联免疫吸附试剂盒定量 LTA。

结果

根管预备前，100%（48/48）的样本中均可回收粪肠球菌 LTA。GWS+MIT 和 GWS+CIT 是针对粪肠球菌 LTA 最有效的方案，两者之间无差异（P>.05）。PUI+CIT 比 PUI+MIT 更有效（P<.05），但比 GWS+MIT 和 GWS+CIT 效果差（P<.05）。GWS 组治疗后未检出粪肠球菌 LTA 的根管数量多于所有测试组。