Department of Otorhinolaryngology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
Department of Otorhinolaryngology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
PLoS One. 2021 Apr 8;16(4):e0249688. doi: 10.1371/journal.pone.0249688. eCollection 2021.
Chronic rhinosinusitis (CRS) is characterized according to the presence or absence of nasal polyps (NPs) and displays nasal microbiota dysbiosis. However, optimal sampling methods of the nasal microbiome in CRS have not been identified. We aimed to assess the microbial composition in patients with CRS, comparing different sampling methods (swab and tissue biopsy), tissue types (uncinate tissue and NP), and disease subtypes. Samples were obtained by swabbing the middle meatus and taking a biopsy of uncinate tissue (UT) in patients with CRS with (CRSwNP, N = 8) or without NP (CRSsNP, N = 6) and controls (N = 8). NPs were also harvested in CRSwNP. DNAs were extracted from fifty-two samples and analyzed by 16S rRNA gene amplicon sequencing. As a result, a great interpersonal variance was observed in nasal swabs, while UT samples presented distinct microbiome with low inter-personal differences. Moreover, the UT microbiomes were further differentiated into three clusters which are associated with disease status (control, CRSsNP, and CRSwNP). Compared to UT, NP revealed a unique microbiome profile with significantly less bacterial diversity. Prevotella was the genus whose abundance was negatively correlated with disease severity in NP. In conclusion, tissue samples are better specimens than nasal swabs for assessing the microbiomes of CRS patients. Several bacteria in UT and NP tissues revealed an association with clinical severity of CRSwNP.
慢性鼻-鼻窦炎（CRS）的特征是存在或不存在鼻息肉（NPs），并表现出鼻腔微生物群落失调。然而，CRS 鼻腔微生物组的最佳采样方法尚未确定。我们旨在通过比较不同的采样方法（拭子和组织活检）、组织类型（钩突组织和 NP）和疾病亚型，评估 CRS 患者的微生物组成。在 CRSwNP（N = 8）或无 NP（CRSsNP，N = 6）的 CRS 患者和对照组（N = 8）中，通过擦拭中鼻甲和钩突组织活检获得样本。在 CRSwNP 中还采集了 NP。从 52 个样本中提取 DNA，并通过 16S rRNA 基因扩增子测序进行分析。结果表明，鼻腔拭子中存在很大的人际差异，而 UT 样本具有明显的微生物组，人际差异较小。此外，UT 微生物组进一步分为三个簇，与疾病状态（对照、CRSsNP 和 CRSwNP）相关。与 UT 相比，NP 显示出独特的微生物组谱，细菌多样性明显较少。普雷沃氏菌属的丰度与 NP 中疾病严重程度呈负相关。总之，组织样本比鼻腔拭子更适合评估 CRS 患者的微生物组。UT 和 NP 组织中的几种细菌与 CRSwNP 的临床严重程度有关。
