Pritchard D G, Stuart F A, Wilesmith J W, Cheeseman C L, Brewer J I, Bode R, Sayers P E
J Hyg (Lond). 1986 Aug;97(1):27-36. doi: 10.1017/s0022172400064329.
Following epidemiological and ecological studies of a defined badger population in an area of East Sussex, removal of all badgers by cage trapping was attempted. Trapping was incomplete due to the activities of protesters. Forty-seven badgers were caught from the eight social groups. All badgers were examined clinically and samples of faeces, urine and tracheal aspirate were taken, together with swabs from any bite wounds, for bacteriological examinations. Forty-five animals were skin tested using whole killed cells of Mycobacterium bovis strain AN5, bovine PPD Weybridge and new human tuberculin. Skin test results were recorded after 24 and 72 h. All badgers were killed and subjected to a post-mortem and bacteriological examination. M. bovis was detected in 10 (21.3%) badgers at post-mortem and in 2 badgers from clinical samples. Four social groups were infected. Positive skin test results were recorded at 72 h with bovine PPD (2 micrograms and 20 micrograms/ml), strain AN5 (1 mg/ml) and human tuberculin (2 micrograms/ml), but not with human tuberculin at 20 micrograms/ml. Histological sections of the skin test reactions showed the cellular types typical of delayed-type hypersensitivity. The skin test reactions observed were neither sensitive nor specific enough to be of practical value.
在对东萨塞克斯郡某一特定獾种群进行流行病学和生态学研究之后，尝试通过笼捕的方式清除该区域内所有的獾。由于抗议者的活动，诱捕并不彻底。从八个社群中捕获了47只獾。对所有獾进行临床检查，并采集粪便、尿液和气管吸出物样本，同时采集任何咬伤伤口的拭子用于细菌学检查。使用牛型结核分枝杆菌菌株AN5的全灭活细胞、韦布里奇牛结核菌素纯蛋白衍生物（PPD）和新型人结核菌素对45只动物进行皮肤试验。在24小时和72小时后记录皮肤试验结果。所有獾均被处死并进行尸检和细菌学检查。尸检时在10只（21.3%）獾中检测到牛型结核分枝杆菌，临床样本中在2只獾中检测到。四个社群受到感染。在72小时时，使用2微克/毫升和20微克/毫升的牛结核菌素纯蛋白衍生物、1毫克/毫升的菌株AN5和2微克/毫升的人结核菌素时皮肤试验结果呈阳性，但使用20微克/毫升的人结核菌素时结果为阴性。皮肤试验反应的组织学切片显示出迟发型超敏反应典型的细胞类型。所观察到的皮肤试验反应既不够敏感也不够特异，没有实际应用价值。
