Centro Andaluz de Biología del Desarrollo, Consejo Superior de Investigaciones Científicas-Universidad Pablo de Olavide-Junta de Andalucía, 41013 Seville, Spain.
Institute of Cellular and Organismic Biology, Academia Sinica, 11529 Taipei, Taiwan.
Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2114802119. doi: 10.1073/pnas.2114802119. Epub 2022 Mar 9.
SignificanceIn this manuscript, we address an essential question in developmental and evolutionary biology: How have changes in gene regulatory networks contributed to the invertebrate-to-vertebrate transition? To address this issue, we perturbed four signaling pathways critical for body plan formation in the cephalochordate amphioxus and in zebrafish and compared the effects of such perturbations on gene expression and gene regulation in both species. Our data reveal that many developmental genes have gained response to these signaling pathways in the vertebrate lineage. Moreover, we show that the interconnectivity between these pathways is much higher in zebrafish than in amphioxus. We conclude that this increased signaling pathway complexity likely contributed to vertebrate morphological novelties during evolution.
在本手稿中，我们探讨了发育和进化生物学中的一个基本问题：基因调控网络的变化如何促成无脊椎动物到脊椎动物的过渡？为了解决这个问题，我们干扰了头索动物文昌鱼和斑马鱼中对身体模式形成至关重要的四条信号通路，并比较了这些干扰对这两个物种中基因表达和基因调控的影响。我们的数据表明，许多发育基因在脊椎动物谱系中获得了对这些信号通路的反应。此外，我们还表明，这些通路之间的相互连接在斑马鱼中比在文昌鱼中要高得多。我们的结论是，这种增加的信号通路复杂性可能有助于脊椎动物在进化过程中的形态新颖性。
