Bonet-Aleta Javier, Garcia-Peiro Jose I, Irusta Silvia, Hueso Jose L
Institute of Nanoscience and Materials of Aragon (INMA), Campus Rio Ebro, CSIC-Universidad de Zaragoza, Edificio I+D, C/Poeta Mariano Esquillor, s/n, 50018 Zaragoza, Spain.
Networking Research Center in Biomaterials, Bioengineering and Nanomedicine (CIBER-BBN), Instituto de Salud Carlos III, 28029 Madrid, Spain.
Nanomaterials (Basel). 2022 Feb 24;12(5):755. doi: 10.3390/nano12050755.
Nanozymes, defined as nanomaterials that can mimic the catalytic activity of natural enzymes, have been widely used to develop analytical tools for biosensing. In this regard, the monitoring of glutathione (GSH), a key antioxidant biomolecule intervening in the regulation of the oxidative stress level of cells or related with Parkinson's or mitochondrial diseases can be of great interest from the biomedical point of view. In this work, we have synthetized a gold-platinum Au@Pt nanoparticle with core-shell configuration exhibiting a remarkable oxidase-like mimicking activity towards the substrates 3,3',5,5'-tetramethylbenzidine (TMB) and -phenylenediamine (OPD). The presence of a thiol group (-SH) in the chemical structure of GSH can bind to the Au@Pt nanozyme surface to hamper the activation of O and reducing its oxidase-like activity as a function of the concentration of GSH. Herein, we exploit the loss of activity to develop an analytical methodology able to detect and quantify GSH up to µM levels. The system composed by Au@Pt and TMB demonstrates a good linear range between 0.1-1.0 µM to detect GSH levels with a limit of detection (LoD) of 34 nM.
纳米酶被定义为能够模拟天然酶催化活性的纳米材料，已被广泛用于开发生物传感分析工具。在这方面，谷胱甘肽（GSH）作为一种关键的抗氧化生物分子，参与细胞氧化应激水平的调节或与帕金森病或线粒体疾病相关，从生物医学角度来看，对其进行监测可能具有重要意义。在这项工作中，我们合成了一种具有核壳结构的金铂Au@Pt纳米颗粒，它对底物3,3',5,5'-四甲基联苯胺（TMB）和邻苯二胺（OPD）表现出显著的类氧化酶模拟活性。GSH化学结构中的巯基（-SH）可与Au@Pt纳米酶表面结合，阻碍O的活化，并根据GSH浓度降低其类氧化酶活性。在此，我们利用活性损失开发了一种分析方法，能够检测和定量高达微摩尔水平的GSH。由Au@Pt和TMB组成的系统在0.1 - 1.0 μM之间显示出良好的线性范围，用于检测GSH水平，检测限（LoD）为34 nM。
