Sun Fujun, Chen Zhiyou, Zhang Qianwei, Wan Yuanyuan, Hu Ran, Shen Shulin, Chen Si, Yin Nengwen, Tang Yunshan, Liang Ying, Lu Kun, Qu Cunmin, Hua Wei, Li Jiana
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.
Plants (Basel). 2022 Feb 28;11(5):667. doi: 10.3390/plants11050667.
The TIFY gene family plays important roles in various plant biological processes and responses to stress and hormones. The chromosome-level genome of the species has been released, but knowledge concerning the TIFY family is lacking in the species. The current study performed a bioinformatics analysis on the TIFY family comparing three diploid (, , and ) and two derived allotetraploid species (, and ). A total of 237 putative TIFY proteins were identified from five species, and classified into ten subfamilies (six JAZ types, one PPD type, two TIFY types, and one ZML type) based on their phylogenetic relationships with proteins in . and species. Duplication and synteny analysis revealed that segmental and tandem duplications led to the expansion of the family genes during the process of polyploidization, and most of these family genes (s) were subjected to purifying selection after duplication based on Ka/Ks values. The spatial and temporal expression patterns indicated that different groups of have distinct spatiotemporal expression patterns under normal conditions and heavy metal stresses. Most of the JAZIII subfamily members were highest in all tissues, but JAZ subfamily members were strongly induced by heavy metal stresses. , , and were significantly upregulated mostly under As and Cd treatment, indicating that they could be actively induced by heavy metal stress. Our results may contribute to further exploration of s, and provided valuable information for further studies of s in plant tolerance to heavy metal stress.
TIFY基因家族在植物的各种生物学过程以及对胁迫和激素的响应中发挥着重要作用。该物种的染色体水平基因组已发布，但关于该物种的TIFY家族的知识却很匮乏。当前研究对TIFY家族进行了生物信息学分析，比较了三个二倍体物种（[具体物种1]、[具体物种2]和[具体物种3]）以及两个衍生的异源四倍体物种（[具体物种4]和[具体物种5]）。从五个[具体物种]中总共鉴定出237个推定的TIFY蛋白，并根据它们与[具体物种]中TIFY蛋白的系统发育关系，将其分为十个亚家族（六种JAZ类型、一种PPD类型、两种TIFY类型和一种ZML类型）。重复和共线性分析表明，片段重复和串联重复导致了多倍体化过程中TIFY家族基因的扩增，并且基于Ka/Ks值，这些TIFY家族基因中的大多数在复制后经历了纯化选择。时空表达模式表明，不同组的TIFY在正常条件和重金属胁迫下具有不同的时空表达模式。大多数JAZIII亚家族成员在所有组织中的表达最高，但JAZ亚家族成员受到重金属胁迫的强烈诱导。[具体基因1]、[具体基因2]、[具体基因3]和[具体基因4]在砷和镉处理下大多显著上调，表明它们可能被重金属胁迫积极诱导。我们的结果可能有助于进一步探索TIFY基因，并为进一步研究TIFY基因在植物对重金属胁迫的耐受性方面提供有价值的信息。
