Gorina Svetlana, Ogorodnikova Anna, Mukhtarova Lucia, Toporkova Yana
Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, P.O. Box 30, 420111 Kazan, Russia.
Plants (Basel). 2022 Mar 2;11(5):683. doi: 10.3390/plants11050683.
The metabolism of polyunsaturated fatty acids through the lipoxygenase-catalyzed step and subsequent reactions is referred to as the lipoxygenase (LOX) pathway. The components of this system, such as jasmonates, are involved in growth, development and defense reactions of plants. In this report, we focus on dynamics of expression of different LOX pathway genes and activities of target enzymes with three abiotic stress factors: darkness, salinity and herbicide toxicity. To obtain a more complete picture, the expression profiles of marker genes for salicylic acid, abscisic acid, ethylene, auxin and gibberellin-dependent signaling systems under the same stresses were also analyzed. The gene expression in plants was analyzed using qRT-PCR, and we found that the LOX-cascade-related genes responded to darkness, salinity and herbicide toxicity in different ways. We detected activation of a number of 9-LOX pathway genes; however, in contrast to studies associated with biotic stress (infection), the 9-divinyl ether synthase branch of the LOX cascade was inhibited under all three stresses. GC-MS analysis of the oxylipin profiles also showed the main activity of the 9-LOX-cascade-related enzymes after treatment with herbicide and darkness.
多不饱和脂肪酸通过脂氧合酶催化步骤及后续反应的代谢过程被称为脂氧合酶（LOX）途径。该系统的组成成分，如茉莉酸类物质，参与植物的生长、发育和防御反应。在本报告中，我们聚焦于不同LOX途径基因的表达动态以及三种非生物胁迫因素（黑暗、盐度和除草剂毒性）作用下目标酶的活性。为了更全面地了解情况，我们还分析了在相同胁迫下，水杨酸、脱落酸、乙烯、生长素和赤霉素依赖信号系统的标记基因的表达谱。利用qRT-PCR分析了植物中的基因表达情况，我们发现与LOX级联相关的基因对黑暗、盐度和除草剂毒性有不同的响应方式。我们检测到多个9-LOX途径基因被激活；然而，与生物胁迫（感染）相关的研究不同，在所有三种胁迫下，LOX级联的9-二乙烯基醚合酶分支均受到抑制。对氧脂素谱的GC-MS分析也表明，在用除草剂处理和黑暗处理后，9-LOX级联相关酶的活性最为显著。
