He Xin, Sun Jiping, Liu Chao, Yu Xiaoyang, Li Huixian, Zhang Wenjing, Li Yan, Geng Yingzhou, Wang Zhigang
Dialysis Department of Nephrology Hospital, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.
Department of Nephrology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.
Diabetes Metab Syndr Obes. 2022 Mar 6;15:755-765. doi: 10.2147/DMSO.S347805. eCollection 2022.
Emerging evidence has revealed that gut microbiota plays a pivotal role in the pathogenesis of type 2 diabetes mellitus (TDM) and diabetic kidney disease (DKD). However, few studies have used metagenomic sequencing to analyze the alterations of gut microbiota community in patients with early-stage DKD.
We carried out metagenomic sequencing in fecal samples of 10 DKD patients (DKD group) and 10 TDM patients who appeared to be less prone to DKD (non-DKD group), aiming to compare the composition and function of gut microbiota between the DKD and non-DKD groups.
The gut microbial community of the DKD group was significantly different from that of the non-DKD group, characterized by a marked increase in phylum Proteobacteria, genus , species, unclassified, and . The amounts of species and were significantly and positively correlated with the urinary albumin creatinine ratio in the DKD group. Furthermore, functional analysis based on dbCAN and KEGG databases showed aberrant lipopolysaccharide (LPS) biosynthesis and carbohydrate metabolism in the gut microbiome of the DKD group.
Our findings provided evidence for alterations in the composition and function of gut microbiota in patients with DKD versus the non-DKD group. These data may contribute to a more comprehensive understanding of the pathological mechanisms of DKD.
新出现的证据表明，肠道微生物群在2型糖尿病（TDM）和糖尿病肾病（DKD）的发病机制中起关键作用。然而，很少有研究使用宏基因组测序来分析早期DKD患者肠道微生物群群落的变化。
我们对10例DKD患者（DKD组）和10例似乎不太容易发生DKD的TDM患者（非DKD组）的粪便样本进行了宏基因组测序，旨在比较DKD组和非DKD组之间肠道微生物群的组成和功能。
DKD组的肠道微生物群落与非DKD组显著不同，其特征是变形菌门、属、种、未分类和的显著增加。DKD组中种和的数量与尿白蛋白肌酐比值呈显著正相关。此外，基于dbCAN和KEGG数据库的功能分析显示，DKD组肠道微生物群中脂多糖（LPS）生物合成和碳水化合物代谢异常。
我们的研究结果为DKD患者与非DKD组相比肠道微生物群的组成和功能变化提供了证据。这些数据可能有助于更全面地了解DKD的病理机制。
