Merlino J, Leroi M, Bradbury R, Veal D, Harbour C
Department of Microbiology and Infectious Diseases, Concord Repatriation General Hospital, Concord 2139, New South Wales, Australia. john.micr.crg.cs.nsw.gov.au
J Clin Microbiol. 2000 Jun;38(6):2378-80. doi: 10.1128/JCM.38.6.2378-2380.2000.
This paper describes a new chromogenic plate medium, CHROMagar Staph aureus (CHROMagar, Paris, France), for the identification of Staphylococcus aureus on the basis of colony pigmentation. The abilities of CHROMagar Staph aureus, thermostable nuclease (DNase), and mannitol salt agar (MSA) to identify S. aureus isolates (n = 114) and discriminate between S. aureus and coagulase-negative staphylococci (CoNS; n = 22) were compared. CHROMagar Staph aureus proved to be more sensitive and specific than DNase and MSA, allowing a reliable, simple, and rapid method for the identification of S. aureus isolates. All CoNS encountered in this study with the exception of S. chromogenes could be easily differentiated from S. aureus on this medium. The supplementation with 4 microgram of oxacillin or methicillin per ml allowed simple identification of methicillin resistance in hospital-acquired S. aureus strains which show multiple-drug resistance profiles. Community-acquired methicillin-resistant S. aureus strains showing non-multi-drug resistance profiles require further evaluation on this new chromogenic medium. Methicillin or oxacillin resistance of all S. aureus isolates was confirmed by the detection of penicillin-binding protein 2a, encoded by the mecA gene, using the latex slide agglutination MRSA-Screen test (PBP 2' Test, DR900M; Oxoid).
本文介绍了一种新的显色平板培养基——金黄色葡萄球菌CHROMagar培养基（CHROMagar，法国巴黎），用于根据菌落色素沉着鉴定金黄色葡萄球菌。比较了金黄色葡萄球菌CHROMagar培养基、耐热核酸酶（DNase）和甘露醇盐琼脂（MSA）对金黄色葡萄球菌分离株（n = 114）的鉴定能力以及区分金黄色葡萄球菌和凝固酶阴性葡萄球菌（CoNS；n = 22）的能力。结果证明，金黄色葡萄球菌CHROMagar培养基比DNase和MSA更敏感、更具特异性，为鉴定金黄色葡萄球菌分离株提供了一种可靠、简单且快速的方法。在本研究中遇到的除产色葡萄球菌外的所有CoNS，在此培养基上都能很容易地与金黄色葡萄球菌区分开来。每毫升添加4微克苯唑西林或甲氧西林，可简单鉴定医院获得性金黄色葡萄球菌菌株中的耐甲氧西林情况，这些菌株表现出多重耐药谱。对于社区获得性耐甲氧西林金黄色葡萄球菌菌株，若其不表现多重耐药谱，则需要在这种新的显色培养基上进一步评估。使用乳胶玻片凝集MRSA-Screen试验（PBP 2'试验，DR900M；Oxoid）检测由mecA基因编码的青霉素结合蛋白2a，从而确认所有金黄色葡萄球菌分离株的甲氧西林或苯唑西林耐药性。
