Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Curr Microbiol. 2022 Mar 14;79(5):129. doi: 10.1007/s00284-022-02820-w.
The pathogenesis of celiac disease (CD) is significantly influenced by gut microbiota. Daily nutritional profile influences the diversity of gut microbiota. This study was aimed to compare the abundance of gut microbiota in CD patients compared to normal control (NC), and to investigate the impact of nutritional factors on their fecal microbiota diversity. In this study, a selected panel of intestinal bacteria was assessed in 31 confirmed CD patients adhering to gluten-free diet (GFD) for more than 6 months and in 20 NC subjects. Stool samples were collected from each participant, DNA was extracted, and absolute quantitative real-time PCR (qPCR) was carried out. The gut microbiota including Bacteroidetes, Bifidobacterium, Clostridium, Staphylococcus, Enterobacteiaceae, Firmicutes, and Lactobacillus were assessed. The quantities of fruits, vegetables, meat, liquids, sugar and gluten-free candy/bread consumption were evaluated using a questionnaire. The proportion of Bifidobacterium, Firmicutes, and Lactobacillus in CD cases was significantly lower than NC (P < 0.005). Significant correlation coefficients between Bifidobacterium and Lactobacillus (P < 0.001), and also Firmicutes and Lactobacillus (P < 0.001) were recorded. Moreover, a significant association between medium amount of meat and bean consumptions and low abundance of Lactobacillus and Firmicutes (P = 0.024 and P = 0.027, respectively), and also high amount of bean consumptions and low abundance of Lactobacillus (P = 0.027) in CD were observed. The results showed that meat and bean consumptions could reduce the beneficial bacteria including Firmicutes and Lactobacillus in CD patients. Therefore, changes in the gut microbiota abundance may contribute to dietary changes and unimproved CD symptoms.
乳糜泻（CD）的发病机制受肠道微生物群显著影响。日常营养状况影响肠道微生物群的多样性。本研究旨在比较 CD 患者与正常对照（NC）肠道微生物群的丰度，并研究营养因素对其粪便微生物多样性的影响。在这项研究中，评估了 31 名坚持无麸质饮食（GFD）治疗超过 6 个月的确诊 CD 患者和 20 名 NC 受试者肠道中选定的一组肠道细菌。从每个参与者收集粪便样本，提取 DNA，并进行绝对定量实时 PCR（qPCR）。评估了肠道微生物群，包括拟杆菌门、双歧杆菌、梭菌、葡萄球菌、肠杆菌科、厚壁菌门和乳杆菌。通过问卷评估水果、蔬菜、肉类、液体、糖和无麸质糖果/面包的摄入量。CD 病例双歧杆菌、厚壁菌门和乳杆菌的比例明显低于 NC（P<0.005）。双歧杆菌和乳杆菌（P<0.001）以及厚壁菌门和乳杆菌（P<0.001）之间存在显著的相关系数。此外，还观察到 CD 中肉类和豆类中等摄入量与乳杆菌和厚壁菌丰度低（P=0.024 和 P=0.027）以及豆类高摄入量与乳杆菌丰度低（P=0.027）之间存在显著相关性。结果表明，肉类和豆类的摄入可能会减少包括厚壁菌门和乳杆菌在内的有益细菌。因此，肠道微生物群丰度的变化可能导致饮食改变和 CD 症状未改善。
