定量杀菌试验的理想靶标生物。
Ideal target organism for quantitative bactericidal assays.
作者信息
Hooke A M, Oeschger M P, Zeligs B J, Bellanti J A
出版信息
Infect Immun. 1978 May;20(2):406-11. doi: 10.1128/iai.20.2.406-411.1978.
Abstract
We have developed a target organism which permits quantitative bactericidal assays. The organism is an Escherichia coli mutant which cannot grow at the temperature of the assay (37 degrees C), but retains full colony-forming potential for subsequent quantitation at 25 degrees C. We show that quantitative data on the bactericidal capacity of polymorphonuclear leukocytes and alveolar macrophages can be obtained when this mutant is used as a target. The procedure used to generate the strain is described in detail and should be applicable to many bacterial species. Characterization of the properties of the mutant indicates that it has a strong potential for use in other in vivo and in vitro investigations of host responses to microbial invasion.
摘要
我们已经培育出一种用于定量杀菌试验的靶标微生物。该微生物是一种大肠杆菌突变体，它在试验温度（37摄氏度）下无法生长，但在25摄氏度下仍保留完整的集落形成能力以便后续进行定量分析。我们表明，当使用这种突变体作为靶标时，可获得关于多形核白细胞和肺泡巨噬细胞杀菌能力的定量数据。详细描述了用于培育该菌株的程序，并且该程序应适用于许多细菌种类。对该突变体特性的表征表明，它在宿主对微生物入侵的其他体内和体外研究中具有很强的应用潜力。
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