Yao Chun-Hsu, Yang Bo-Yin, Li Yi-Chen Ethan
School of Chinese Medicine, College of Chinese Medicine, Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan.
Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan.
Front Bioeng Biotechnol. 2022 Feb 28;10:832808. doi: 10.3389/fbioe.2022.832808. eCollection 2022.
The regeneration defect of bone is a long-term physiological process after bone injuries. To accelerate the bone remodeling process, the combination of chemical and physical stimulations provides an efficient strategy to allow maturation and to functionalize osteoclasts and osteoblasts. This study aims to investigate the dual effects of a tricalcium phosphate (TCP)-based gelatin scaffold (GGT) in combination with electroacupuncture stimulation on the activation of osteoclasts and osteoblasts, as well as new bone regrowth . We demonstrated that electrical stimulation changes the pH of a culture medium and activates osteoblasts and osteoclasts in an co-culture system. Furthermore, we showed that electroacupuncture stimulation can enhance osteogenesis and new bone regrowth and can upregulate the mechanism among parathyroid hormone intact (PTH-i), calcium, osteoclasts, and osteoblasts in the bone-defected rats. Those results showed the potential interest to combine the electroacupuncture technique with GGT scaffolds to improve bone remodeling after injury.
骨再生缺陷是骨损伤后的一个长期生理过程。为加速骨重塑过程，化学和物理刺激的联合提供了一种有效的策略，可促使破骨细胞和成骨细胞成熟并发挥功能。本研究旨在探究基于磷酸三钙（TCP）的明胶支架（GGT）与电针刺激联合对破骨细胞和成骨细胞激活以及新骨再生的双重作用。我们证明电刺激可改变培养基的pH值，并在共培养系统中激活成骨细胞和破骨细胞。此外，我们表明电针刺激可增强骨生成和新骨再生，并能上调骨缺损大鼠体内甲状旁腺激素完整形式（PTH-i）、钙、破骨细胞和成骨细胞之间的作用机制。这些结果显示了将电针技术与GGT支架相结合以改善损伤后骨重塑的潜在价值。
