Özdemir Selçuk, Arslan Harun
Atatürk University, Faculty of Veterinary Medicine, Department of Genetics, Erzurum, Turkey; Heinrich Heine University, Faculty of Medicine, Department of Gastroenterology, Hepatology, and Infection, Düsseldorf, Germany.
Atatürk University, Faculty of Fisheries, Department of Basic Science, Erzurum, Turkey.
Chemosphere. 2022 Jul;298:134330. doi: 10.1016/j.chemosphere.2022.134330. Epub 2022 Mar 15.
Circular RNAs (circRNAs) are a new class of non-endogenous coding RNA and an area with a lot of research interest and activity. Cypermethrin and chlorpyrifos have been shown to cause serious toxicological damage in the brain of fish and other non-target organisms. However, circRNAs associated with acute brain toxicity caused by cypermethrin and chlorpyrifos have not been studied yet. In this study, circRNAs were identified and characterized using RNA-seq in Zebrafish brains exposed to acute cypermethrin and chlorpyrifos toxicity. A total of 10,375 circRNAs were detected. It was determined that 6 circRNAs were up-regulated, 10 circRNAs were down-regulated in CYP brain samples compared to controls. In addition, it was found that 57 circRNAs are up-regulated and 3 circRNAs down-regulated in CPF brain samples compared to controls. Moreover, 62 circRNAs were down-regulated in the CYP samples, when CYP and CPF samples were compared. However, up-regulated circRNA could not be detected. It was revealed that the detected circRNAs specifically regulated the MAPK signaling pathway, endocytosis mechanism, apoptosis, and p53 signaling pathway. This study, which was conducted for the first time in terms of the subject of the study, could bring a different perspective, especially to pesticide toxicity studies.
环状RNA（circRNAs）是一类新型的非内源性编码RNA，是一个具有大量研究兴趣和活动的领域。氯氰菊酯和毒死蜱已被证明会对鱼类和其他非靶标生物的大脑造成严重的毒理学损害。然而，与氯氰菊酯和毒死蜱引起的急性脑毒性相关的环状RNA尚未得到研究。在本研究中，利用RNA测序在暴露于急性氯氰菊酯和毒死蜱毒性的斑马鱼大脑中鉴定并表征了环状RNA。共检测到10375个环状RNA。结果表明，与对照组相比，CYP脑样本中有6个环状RNA上调，10个环状RNA下调。此外，与对照组相比，CPF脑样本中有57个环状RNA上调，3个环状RNA下调。此外，当比较CYP和CPF样本时，CYP样本中有62个环状RNA下调。然而，未检测到上调的环状RNA。结果显示，检测到的环状RNA特异性调节丝裂原活化蛋白激酶信号通路、内吞作用机制、细胞凋亡和p53信号通路。本研究在研究主题方面首次进行，尤其可为农药毒性研究带来不同的视角。
