Department of Genetics, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey; German Center for Neurodegenerative Diseases, DZNE, Bonn, Germany.
Department of Genetics, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey.
Chemosphere. 2023 Dec;344:140324. doi: 10.1016/j.chemosphere.2023.140324. Epub 2023 Sep 29.
Pesticides, such as cypermethrin (CYP) and chlorpyrifos (CPF), are widely used around the world and are known to cause toxicological effects in the brains of fish and other non-target organisms. Long non-coding RNAs (LncRNAs) are a new class of non-coding RNAs that are highly expressed in the brain and play crucial roles in brain function by regulating gene expression. Many studies have investigated the toxic effects of CYP and CPF on the brain. However, no study has been conducted on the relationship between LncRNAs and the toxicity caused by these chemicals. Therefore, this study aimed to determine changes in the lncRNA expression profile in the brains of fish exposed to CYP and CPF. Out of a total of 482 lncRNAs that were differentially expressed between control and CPF groups, 53 were found to be up-regulated, and 429 were down-regulated. Similarly, among the 200 lncRNAs differentially expressed between the control and CYP groups, 71 were up-regulated, and 129 were down-regulated. Additionally, 268 differentially expressed lncRNAs were identified between CYP and CPF groups, with 240 being up-regulated and the rest being down-regulated. In addition, LncRNAs expressed from fish brains exposed to CYP and CPF were found to regulate multiple signaling pathways, including MAPK, FoxO, PPAR, TGF-β, and Wnt signaling pathways.
农药，如氯菊酯（CYP）和毒死蜱（CPF），在世界范围内广泛使用，已知会对鱼类和其他非靶标生物的大脑造成毒理学影响。长链非编码 RNA（LncRNA）是一类新的非编码 RNA，在大脑中高度表达，通过调节基因表达在大脑功能中发挥关键作用。许多研究已经调查了 CYP 和 CPF 对大脑的毒性作用。然而，尚未研究 LncRNA 与这些化学物质引起的毒性之间的关系。因此，本研究旨在确定暴露于 CYP 和 CPF 的鱼类大脑中的 lncRNA 表达谱变化。在对照组和 CPF 组之间差异表达的总共 482 个 lncRNA 中，有 53 个上调，429 个下调。同样，在对照组和 CYP 组之间差异表达的 200 个 lncRNA 中，有 71 个上调，129 个下调。此外，在 CYP 和 CPF 组之间鉴定出 268 个差异表达的 lncRNA，其中 240 个上调，其余下调。此外，从暴露于 CYP 和 CPF 的鱼类大脑中表达的 LncRNA 被发现调节多个信号通路，包括 MAPK、FoxO、PPAR、TGF-β和 Wnt 信号通路。
