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用于风疹抗体检测的单径向溶血(SRH)技术评估。

Evaluation of the single radial haemolysis (SRH) technique for rubella antibody measurement.

作者信息

Russell S M, Benjamin S R, Briggs M, Jenkins M, Mortimer P P, Payne S B

出版信息

J Clin Pathol. 1978 Jun;31(6):521-6. doi: 10.1136/jcp.31.6.521.

Abstract

Sera from 1258 individuals have been tested by four laboratories for rubella antibody by both the haemagglutination-inhibition and single radial haemolysis techniques. There was good agreement between the results obtained by the two methods. Although sheep red blood cells were used in the single radial haemolysis plates, no problems were encountered with sera from patients with infectious mononucleosis. The single haemolysis technique was found to be simple, convenient, and reliable, and suited to the rapid screening of large numbers of sera to assess susceptibility to rubella in the context of a vaccination campaign. However, since the technique does not detect anti-rubella IgM, it should not be used as the only test to investigate suspected recent infection.

摘要

来自1258名个体的血清已由四个实验室采用血凝抑制试验和单向辐射溶血技术检测风疹抗体。两种方法所得结果吻合良好。尽管单向辐射溶血试验平板中使用了绵羊红细胞,但传染性单核细胞增多症患者的血清未出现问题。单向溶血技术简单、便捷、可靠,适合在疫苗接种活动中快速筛查大量血清以评估风疹易感性。然而,由于该技术无法检测抗风疹IgM,因此不应作为调查疑似近期感染的唯一检测方法。

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