三种风疹抗体筛查检测方法的比较。
A comparison of three tests for rubella antibody screening.
作者信息
Morgan-Capner P, Pullen H J, Pattison J R, Bidwell D E, Bartlett A, Voller A
出版信息
J Clin Pathol. 1979 Jun;32(6):542-5. doi: 10.1136/jcp.32.6.542.
Abstract
Sera from 1000 women attending antenatal clinics were screened for the presence of rubella antibody by haemagglutination inhibition (HI), single radial haemolysis (SRH), and enzyme-linked immunosorbent assay (ELISA). With 925 sera concordant results were obtained with all three tests. There were 46 false-negative HI tests due to the necessity of allowing for residual non-specific inhibitors. With SRH there was one false positive and three that were negative by SRH but HI- and ELISA-positive. By ELISA 14 sera gave a false-positive result (OD 405 nm greater than or equal to 0.2). Ten of these could be eliminated by taking an OD 405 nm of 0.5 as the threshold but then another 10 sera became false negatives.
摘要
通过血凝抑制试验（HI）、单向辐射溶血试验（SRH）和酶联免疫吸附测定（ELISA）对1000名前往产前诊所就诊的女性的血清进行风疹抗体筛查。在925份血清中，这三种检测方法均得到了一致的结果。由于需要考虑残留的非特异性抑制剂，HI试验出现了46例假阴性结果。SRH试验有1例假阳性，还有3份血清SRH试验为阴性，但HI试验和ELISA试验为阳性。ELISA试验中有14份血清出现假阳性结果（405nm波长处的光密度（OD）大于或等于0.2）。将OD 405nm为0.5作为阈值可消除其中10份，但又有另外10份血清变为假阴性。
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Rubella-virus hemagglutination-inhibition test.风疹病毒血凝抑制试验
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A simple method for removal of rubella hemagglutination inhibitors from serum adaptable to finger-tip blood.一种从血清中去除风疹血凝抑制物的简单方法，适用于指尖血。
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The derivation of a minimum immune titre of rubella haemagglutination-inhibition (HI) antibody. A Public Health Laboratory Service collaborative survey.风疹血凝抑制（HI）抗体最低免疫滴度的推导。一项公共卫生实验室服务合作调查。
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Evaluation of the single radial haemolysis (SRH) technique for rubella antibody measurement.用于风疹抗体检测的单径向溶血（SRH）技术评估。
J Clin Pathol. 1978 Jun;31(6):521-6. doi: 10.1136/jcp.31.6.521.
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An assessment of radial haemolysis in the detection of rubella antibody.检测风疹抗体时的径向溶血评估。
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