Nanditha C K, Kumar G S Vinod
Nano Drug Delivery Systems (NDDS), Bio-Innovation Center (BIC), Rajiv Gandhi Centre for Biotechnology, Thycaud P.O, Thiruvananthapuram, Kerala, 695014, India.
Research Centre, University of Kerala, Thiruvananthapuram, Kerala, India.
Mater Today Bio. 2022 Mar 8;14:100235. doi: 10.1016/j.mtbio.2022.100235. eCollection 2022 Mar.
Hard to heal wounds such as diabetic wounds is one of the major problems in the healthcare sector. Delayed healing and shortfall of functional restoration at the wound site require upgraded wound management aids. In this study, we report that a nanofibrous mat enriched with bioactive peptides laden nano and microparticles achieve the requirements as an effective diabetic wound dressing. By means of electrospinning method, we fabricated Poly (lactic--glycolic acid)/Collagen nano-scale mat and surface functionalised with wound healing peptides, laden Chitosan nano and micro-sized particles, creating an Extracellular Matrix (ECM) -like structure with biomimetic features. The developed dressing displayed good cytocompatibility with Keratinocyte and fibroblast cells and enhanced their cell proliferation and migration. Experiments in the streptozotocin-induced diabetic mice model showed that bioactive peptides released from Chitosan particles shorten the inflammatory stage and promote neovascularisation. The supporting nanoscale matrix promotes increased collagen deposition in the wound beds, thereby hastening the complete healing process by substantial tissue re-generation and functional restoration. The results evince that the nano/microparticles enriched nano-scale mat show potential as an effective wound repair dressing for diabetic wounds.
难愈合伤口，如糖尿病伤口，是医疗保健领域的主要问题之一。伤口愈合延迟和伤口部位功能恢复不足需要升级的伤口管理辅助手段。在本研究中，我们报告了一种富含负载生物活性肽的纳米和微粒的纳米纤维垫可满足作为有效糖尿病伤口敷料的要求。通过静电纺丝法，我们制备了聚（乳酸-乙醇酸）/胶原蛋白纳米级垫，并使用伤口愈合肽进行表面功能化，负载壳聚糖纳米和微米级颗粒，形成具有仿生特征的细胞外基质（ECM）样结构。所开发的敷料与角质形成细胞和成纤维细胞显示出良好的细胞相容性，并增强了它们的细胞增殖和迁移。在链脲佐菌素诱导的糖尿病小鼠模型中的实验表明，壳聚糖颗粒释放的生物活性肽缩短了炎症期并促进了新血管形成。支撑性纳米级基质促进伤口床中胶原蛋白沉积增加，从而通过大量组织再生和功能恢复加速完全愈合过程。结果表明，富含纳米/微粒的纳米级垫显示出作为糖尿病伤口有效伤口修复敷料的潜力。
