Evaluation of anti-gastric ulcer activity of aqueous and 80% methanol leaf extracts of in rats.

BACKGROUND

Gastric ulcer is a major public health problem globally and associated with severe complications including hemorrhages, perforations, gastrointestinal obstruction, and malignancy. is widely used for traditional management of gastric ulcer in different parts of Ethiopia. The present study was undertaken to evaluate the anti-gastric ulcer activity of aqueous and 80% methanol extracts of

METHODS

The leaf extracts were prepared using decoction (aqueous) and maceration (80% methanol) techniques and in anti-gastric ulcer effects of various doses of extracts and the effect were determined using the pylorus ligation, indomethacin and ethanol induced gastric ulcer models.

RESULTS

In pylorus ligation induced gastric ulcer model, both aqueous and 80% methanol extracts at doses of 200 and 400 mg/kg were exhibited significant reduction in total acidity, volume of gastric secretion (p < 0.001) and substansial rise in pH (p˂0.05) of the gastric secretion. In indomethacin induced ulcer model, both aqueous and methanol extracts were exhibited dose dependent increment in gastric wall mucus compared to control (p < 0.001). In ethanol induced ulcer model, all doses of extract produced significant increment in gastric wall mucus from 46.66 ± 0.96 (AQ100) to 75.87 ± 1.52 (ME 400) μg alcian blue/g wet stomach. Five days pre-treatment with 200 mg/kg of both and aqueous and methanolic extracts exhibited significant ( < 0.001) ulcer inhibition in both indomethacin and ethanol-induced ulcer models.

CONCLUSION

Both extracts of exhibited a promising anti-gastric ulcer activity in all of the three models and this findings supports for traditional claimed use of the leaf of