*Department of Orthopedics and Traumatology, Ankara City Hospital-Neurology Orthopaedic Hospital, Ankara, Turkey.
†Department of Orthopedics and Traumatology, Recep Tayyip Erdoğan University Medical School, Rize, Turkey.
J Am Podiatr Med Assoc. 2022 Mar 16;112(1):20-055. doi: 10.7547/20-055.
Adhesions after tendinopathy in individuals who perform physical work and those physically active in middle age are a challenging problem for orthopedic surgeons. We evaluated the effects of human-derivated amniotic membrane on tendon healing, adhesions, angiogenesis, and the inflammatory process.
Thirty-five rats were divided evenly into five groups, and the left lower extremity was used in this study. No interventions were applied to the control group (group 5). In the other groups, Achilles tendons were partially cut to the midline. Then, primary repair (group 1), amniotic membrane treatment with no repair (group 2), primary repair and amniotic membrane treatment (group 3), or secondary healing with no repair (group 4) was performed.
Use of amniotic membrane in tendon healing resulted in decreased adhesion formation and positive effects on collagen sequencing and anti-inflammatory effects. In addition, for the vascular endothelial growth factor evaluation there was no difference among the amniotic membrane repair groups, but there was an increase in vascular endothelial growth factor positivity compared with the control group.
These data show that amniotic membrane treatment can alter biological behavior and induce surface-dependent angiogenesis and can have angiogenetic effects on ischemia and inflammation.
在从事体力工作和中年时身体活跃的人群中，肌腱病后的粘连是骨科医生面临的一个挑战。我们评估了人源性羊膜对肌腱愈合、粘连、血管生成和炎症过程的影响。
35 只大鼠平均分为 5 组，本研究使用左下肢。对照组（第 5 组）不进行任何干预。在其他组中，将跟腱从中线部分切断。然后，进行初次修复（第 1 组）、无修复的羊膜处理（第 2 组）、初次修复和羊膜处理（第 3 组）或无修复的二次愈合（第 4 组）。
羊膜在肌腱愈合中的应用可减少粘连形成，并对胶原序列和抗炎作用产生积极影响。此外，在血管内皮生长因子评估中，羊膜修复组之间没有差异，但与对照组相比，血管内皮生长因子阳性率增加。
这些数据表明，羊膜处理可以改变生物行为，诱导表面依赖性血管生成，并对缺血和炎症具有血管生成作用。
