Iwai Kenta, Iwai-Shimada Miyuki, Asato Kaname, Nakai Kunihiko, Kobayashi Yayoi, Nakayama Shoji F, Tatsuta Nozomi
Health and Environmental Risk Division, National Institute for Environmental Studies, Tsukuba 305-8506, Japan.
Department of Development and Environmental Medicine, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan.
Toxics. 2022 Feb 25;10(3):109. doi: 10.3390/toxics10030109.
For infants in the first months of life, breast milk is a complete source of nutrition; however, it can also contain elements that are harmful to the infant. It is therefore critical for infant health to characterize breast milk. The aim of this study was to determine the intra- and inter-day variation of elements in breast milk, for which there is currently limited information, as a pilot study for a larger study. Firstly, we developed a simple and robust analytical method for the determination of multiple elements in breast milk. It was accurate (accuracy ranged from 98% to 107%) for measurement of 26 elements in breast milk by quadrupole inductively coupled plasma-mass spectrometry. Intra- and inter-day variation of elements, protein, and fat in breast milk was determined by analyzing breast milk collected from 11 women at 12 sampling points over three days and calculating intraclass correlation coefficients. Intraclass correlation coefficients showed that while some elements were consistent across time points (e.g., Sr, Ca, and Cu), others showed very high variability (e.g., As, Cd, and Ni). Correlation analyses between elements in breast milk showed strong relationships between those including Fe and Mo, Ca and Sr, and Cd and Fe.
对于出生后最初几个月的婴儿来说，母乳是一种完整的营养来源；然而，母乳中也可能含有对婴儿有害的成分。因此，对母乳进行特性分析对婴儿健康至关重要。本研究的目的是确定母乳中元素的日内和日间变化情况，目前这方面的信息有限，本研究作为一项更大规模研究的前期研究。首先，我们开发了一种简单而稳健的分析方法来测定母乳中的多种元素。通过四极杆电感耦合等离子体质谱法测定母乳中26种元素时，该方法准确（准确度在98%至107%之间）。通过分析11名女性在三天内12个采样点采集的母乳，并计算组内相关系数，确定了母乳中元素、蛋白质和脂肪的日内和日间变化。组内相关系数表明，虽然某些元素在各时间点保持一致（如锶、钙和铜），但其他元素显示出非常高的变异性（如砷、镉和镍）。母乳中元素之间的相关性分析表明，铁和钼、钙和锶以及镉和铁之间存在很强的相关性。
